Creatine Phosphokinase antibody | BGN/2ba6

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Mouse anti Human Creatine Phosphokinase (BB)

Product Type
Monoclonal Antibody
Clone
BGN/2ba6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2400-1942 E 0.2 mg
Mouse anti human creatine phosphokinase, clone BGN/2ba6 recognizes creatine phosphokinase. Also known as creatine kinase (CK), it is a dimer with a molecular mass of approximately 80 kDa. In vertebrate cells, the cytosolic CK enzymes consist of two different subunits, either B (brain type) or M (muscle type). They combine to produce three different isoenzymes: CKMM, CKBB and CKMB. CKBB is the major CK isoenzyme of the brain (Eppenberger et al, 1967, Dawson et al, 1967).

Creatine phosphokinase is an enzyme expressed in tissues and cell types with high energy requirements and is involved in cellular energy homeostasis. CK reversibly catalyzes the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP) (Wallimann et al, 1992, 2011).

Mouse anti human creatine phosphokinase is specific for the CKBB isoenzyme and does not react with the B subunit in CKMB. There is minimal reactivity with other human serum proteins.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human CKBB
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12277 Related reagents
Entrez Gene
CKB Related reagents
GO Terms
GO:0004111 creatine kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006600 creatine metabolic process
Regulatory
For research purposes only

Applications of Creatine Phosphokinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. Wallimann, T. et al. (2011) The creatine kinase system and pleiotropic effects of creatine.
    Amino Acids. 40(5):1271-96.
  2. Wallimann, T. et al. (1992) Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis.
    Biochem J. 281: 21-40.
  3. Eppenberger, H.M. et al. (1967) The comparative enzymology of creatine kinases. I. Isolation and characterization from chicken and rabbit tissues.
    J Biol Chem. 242: 204-209.
  4. Dawson, D.M. et al. (1967) The comparative enzymology of creatine kinases. II. Physical and chemical properties.
    J Biol Chem. 242: 210-217.