Cranin antibody | 6C1 (4G2)

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Mouse anti Human Cranin

Product Type
Monoclonal Antibody
Clone
6C1 (4G2)
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0074 C E 0.1 ml
Mouse anti Human Cranin antibody, clone 6C1 recognises human cranin, also known as dystroglycan. Cranin is expressed in brain and many other tissues, it binds laminin with high affinity in a calcium dependent manner. It appears to be important in maintaining normal muscle integrity. Loss of cranin from the muscle surface may be one of the primary events leading to muscle injury in congenital muscular dystrophies, it may also play a role in the cognitive deficits often seen in such conditions. Mouse anti Human cranin, clone 6C1 recognizes an extracellular region close to the C terminal end of the alpha subunit of human cranin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Xenopus
Chicken
Sheep
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites - liquid
Preservative Stabilisers
None present
Immunogen
A synthetic peptide corresponding to amino acids 572-604 of human cranin (dystroglycan).

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q14118 Related reagents
Entrez Gene
DAG1 Related reagents
GO Terms
GO:0000299 integral to membrane of membrane fraction
GO:0005509 calcium ion binding
GO:0003779 actin binding
GO:0016021 integral to membrane
GO:0005604 basement membrane
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005856 cytoskeleton
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0008307 structural constituent of muscle
GO:0015631 tubulin binding
GO:0017166 vinculin binding
GO:0030027 lamellipodium
GO:0030175 filopodium
GO:0030336 negative regulation of cell migration
GO:0034453 microtubule anchoring
GO:0043237 laminin-1 binding
GO:0043409 negative regulation of MAPKKK cascade
GO:0044419 interspecies interaction between organisms
GO:0051393 alpha-actinin binding
GO:0051898 negative regulation of protein kinase B signaling cascade
GO:0070938 contractile ring
Regulatory
For research purposes only

Applications of Cranin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/1 1/50
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Neurons, astrocytes, smooth muscle, fibroblasts or epithelial cell lines.

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for Cranin antibody

  1. Smalheiser, N.R. & Kim, E. (1995) Purification of cranin, a laminin binding membrane protein. Identity with dystroglycan and reassessment of its carbohydrate moieties.
    J Biol Chem. 270 (25): 15425-33.
  2. Smalheiser, N.R. & Schwartz, N.B. (1987) Cranin: a laminin-binding protein of cell membranes.
    Proc Natl Acad Sci U S A. 84 (18): 6457-61.