Corticotrophin Releasing Factor antibody | 4H9

Mouse anti Human Corticotrophin Releasing Factor

Product Type
Monoclonal Antibody
Clone
4H9
Isotype
IgG1
Specificity
Corticotrophin Releasing Factor

Product Code Applications Pack Size List Price Your Price Qty
2329-9907
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P * 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.

The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.

Target Species
Human
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
Affinity
Averages 10(e)-9 M with native and modified CRF.
Approx. Protein Concentrations
1mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/100 1/7000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Corticotrophin Releasing Factor antibody

  1. Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.
    Hybridoma. 13(1): 59-64.
  2. DeBellis, A. et al. (2012) Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells.
    J Clin Endocrinol Metab. 97 (10): 3684-90.

Synonyms
Corticoliberin
CRF
RRID
AB_620120
UniProt
P06850
Entrez Gene
CRH
GO Terms
GO:0005576 extracellular region
GO:0007165 signal transduction
GO:0005184 neuropeptide hormone activity
GO:0005625 soluble fraction
GO:0007268 synaptic transmission
GO:0007565 female pregnancy
GO:0007567 parturition
GO:0010841 positive regulation of circadian sleep/wake cycle, wakefulness
GO:0042322 negative regulation of circadian sleep/wake cycle, REM sleep
GO:0051464 positive regulation of cortisol secretion