Corticotrophin Releasing Factor antibody | 4H9
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Mouse anti Human Corticotrophin Releasing Factor
- Product Type
- Monoclonal Antibody
- Clone
- 4H9
- Isotype
- IgG1
- Specificity
- Corticotrophin Releasing Factor
Antibody Quality and 
Unrivaled 
| Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland. The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF. |
- Target Species
- Human
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
- Affinity
- Averages 10(e)-9 M with native and modified CRF.
- Approx. Protein Concentrations
- 1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
1/100 | 1/7000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Corticotrophin Releasing Factor antibody
-
Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.
Hybridoma. 13(1): 59-64. -
DeBellis, A. et al. (2012) Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells.
J Clin Endocrinol Metab. 97 (10): 3684-90.
- Synonyms
- Corticoliberin
- CRF
- RRID
- AB_620120
- UniProt
- P06850
- Entrez Gene
- CRH
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005184 neuropeptide hormone activity
- GO:0005625 soluble fraction
- GO:0007268 synaptic transmission
- GO:0007565 female pregnancy
- GO:0007567 parturition
- GO:0010841 positive regulation of circadian sleep/wake cycle, wakefulness
- GO:0042322 negative regulation of circadian sleep/wake cycle, REM sleep
- View More GO Terms
- GO:0051464 positive regulation of cortisol secretion
View more products with CORTICOTROPHIN RELEASING FACTOR specificity
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