Cortactin antibody

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Mouse anti Cortactin

Mouse anti human cortactin antibody recognizes the Src kinase substrate cortactin, also known as amplaxin, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) or oncogene EMS1. This antibody has been validated for western blotting using whole cell lysates.

Cortactin Antibody with Control Lysate

Mouse anti human cortactin antibody recognizes the Src kinase substrate cortactin, also known as CTTN, 1110020L01Rik, amplaxin, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) or oncogene EMS1.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00417 WB 100 µl
VMA00417KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cortactin antibody recognizes the Src kinase substrate cortactin, also known as CTTN, amplaxin, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) and oncogene EMS1.

The CTTN gene product is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The CTTN protein is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of CTTN contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for CTTN (provided by RefSeq, May 2010).

Mouse anti Human cortactin antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cortactin detects a band of approximately 85 kDa in MCF7 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human cortactin produced in HEK293 cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q14247 Related reagents
Entrez Gene
CTTN Related reagents
GO Terms
GO:0005515 protein binding
GO:0001726 ruffle
GO:0005625 soluble fraction
GO:0005856 cytoskeleton
GO:0005938 cell cortex
GO:0030027 lamellipodium
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cortactin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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