Cortactin antibody

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Anti Cortactin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human cortactin antibody (**VMA00417**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 11 second exposure. Arrow points to cortactin (molecular weight 85 kDa).

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Mouse anti Cortactin

Product Type: PrecisionAb Monoclonal
Isotype: IgG2a
Format: Purified
Specificity: Cortactin

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VMA00417 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human cortactin antibody recognizes the Src kinase substrate cortactin, also known as CTTN, amplaxin, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) and oncogene EMS1.

The CTTN gene product is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The CTTN protein is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of CTTN contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for CTTN (provided by RefSeq, May 2010).

Mouse anti Human cortactin antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cortactin detects a band of approximately 85 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Human cortactin produced in HEK293 cells
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14247
Entrez Gene: CTTN
GO Terms: GO:0005515 protein binding; GO:0001726 ruffle; GO:0005625 soluble fraction; GO:0005856 cytoskeleton; GO:0005938 cell cortex; GO:0030027 lamellipodium