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Coro1A antibody | OTI1A5

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Anti Coro1A Antibody, clone OTI1A5 (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Coro1A Antibody, clone OTI1A5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human CORO1A antibody, clone OTI1A5 (VMA00283) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to CORO1A (molecular weight 51 kDa).

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WB

Mouse anti Coro1A

Product Type
PrecisionAb Monoclonal
Clone
OTI1A5
Isotype
IgG1
Format
Purified
Specificity
Coro1A

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VMA00283
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WB 100 µl loader
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Mouse anti Human CORO1A antibody, clone OTI1A5 recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-1A, coronin-like protein A, coronin-like protein p57 and tryptophan aspartate-containing coat protein.

Encoded by the CORO1A gene, coronin-1A is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16 (provided by RefSeq, Sep 2010).

Mouse anti Human CORO1A antibody, clone OTI1A5 detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CORO1A detects a band of approximately 51 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length ecombinant human CORO1A (NP_009005) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P31146
Entrez Gene
CORO1A
GO Terms
GO:0001845 phagolysosome assembly
GO:0001891 phagocytic cup
GO:0051015 actin filament binding
GO:0008022 protein C-terminus binding
GO:0030027 lamellipodium
GO:0030670 phagocytic vesicle membrane
GO:0030864 cortical actin cytoskeleton
GO:0031589 cell-substrate adhesion
GO:0031941 filamentous actin
GO:0042803 protein homodimerization activity
GO:0043548 phosphatidylinositol 3-kinase binding
GO:0045087 innate immune response
GO:0050918 positive chemotaxis
GO:0051126 negative regulation of actin nucleation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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