Coro1A antibody | OTI1A5
Mouse anti Coro1A
The CORO1A antibody recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-like protein A, coronin-like protein p57 or tryptophan aspartate-containing coat protein. This antibody has been extensively validated for western blotting using whole cell lysates.
Coro1A Antibody with Control Lysate
The CORO1A antibody recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-like protein A, coronin-like protein p57 or tryptophan aspartate-containing coat protein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1A5
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00283 | WB | 100 µl | ||||
| VMA00283KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human CORO1A antibody recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-1A, coronin-like protein A, coronin-like protein p57 and tryptophan aspartate-containing coat protein.
Encoded by the CORO1A gene, coronin-1A is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16 (provided by RefSeq, Sep 2010).
Mouse anti Human CORO1A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the CORO1A gene, coronin-1A is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16 (provided by RefSeq, Sep 2010).
Mouse anti Human CORO1A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CORO1A detects a band of approximately 51 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length ecombinant human CORO1A (NP_009005) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P31146 Related reagents
- Entrez Gene
- CORO1A Related reagents
- GO Terms
- GO:0001845 phagolysosome assembly
- GO:0001891 phagocytic cup
- GO:0051015 actin filament binding
- GO:0008022 protein C-terminus binding
- GO:0030027 lamellipodium
- GO:0030670 phagocytic vesicle membrane
- GO:0030864 cortical actin cytoskeleton
- GO:0031589 cell-substrate adhesion
- GO:0031941 filamentous actin
- GO:0042803 protein homodimerization activity
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0045087 innate immune response
- GO:0050918 positive chemotaxis
- GO:0051126 negative regulation of actin nucleation
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Coro1A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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