Coro1A antibody | OTI1A5

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Anti Coro1A Antibody, clone OTI1A5 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CORO1A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. Arrow points to CORO1A (molecular weight 51 kDa)

Mouse anti Coro1A

The CORO1A antibody recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-like protein A, coronin-like protein p57 or tryptophan aspartate-containing coat protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Coro1A Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1A5
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00283	WB	100 µl
VMA00283KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human CORO1A antibody recognizes coronin-1A, also known as clipin-A, coronin-1, coronin-1A, coronin-like protein A, coronin-like protein p57 and tryptophan aspartate-containing coat protein.

Encoded by the CORO1A gene, coronin-1A is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16 (provided by RefSeq, Sep 2010).

Mouse anti Human CORO1A antibody detects a band of 51 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length ecombinant human CORO1A (NP_009005) produced in HEK293T cells

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P31146 Related reagents
Entrez Gene: CORO1A Related reagents
GO Terms: GO:0001845 phagolysosome assembly; GO:0001891 phagocytic cup; GO:0051015 actin filament binding; GO:0008022 protein C-terminus binding; GO:0030027 lamellipodium; GO:0030670 phagocytic vesicle membrane; GO:0030864 cortical actin cytoskeleton; GO:0031589 cell-substrate adhesion; GO:0031941 filamentous actin; GO:0042803 protein homodimerization activity; GO:0043548 phosphatidylinositol 3-kinase binding; GO:0045087 innate immune response; GO:0050918 positive chemotaxis; GO:0051126 negative regulation of actin nucleation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Coro1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti CORO1A detects a band of approximately 51 kDa in Jurkat cell lysates
Western Blotting: Anti CORO1A detects a band of approximately 51 kDa in Jurkat cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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