Complement Factor P antibody | 10-18

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Mouse anti Human Factor P

Product Type
Monoclonal Antibody
Clone
10-18
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2617 C E FN WB 0.1 mg
Mouse anti Human Factor P antibody, clone 10-18 recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections.

Mouse anti Human Factor P antibody, clone 10-18 is reported to inhibit the function of factor P. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Native purified factor P from human serum.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date

More Information

UniProt
P27918 Related reagents
Entrez Gene
CFP Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0005615 extracellular space
GO:0006957 complement activation, alternative pathway
Regulatory
For research purposes only

Applications of Complement Factor P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney from post streptoccal glomerulonephritis patients

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. Hourcade, D.E. (2006) The role of properdin in the assembly of the alternative pathway C3 convertases of complement.
    J Biol Chem. 281 (4): 2128-32.
  2. Jelezarova, E. et al. (2000) Interaction of C3b(2)--IgG complexes with complement proteins properdin, factor B and factor H: implications for amplification.
    Biochem J. 349 (Pt 1): 217-23.