COMMD1 antibody | OTI3B3

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Mouse anti COMMD1

COMMD1 antibody recognizes the COMM domain-containing protein 1, also known as MURR1. The antibody has been extensively validated for western blotting using whole cell lysates.

COMMD1 Antibody with Control Lysate

COMMD1 antibody recognizes the COMM domain-containing protein 1, also known as MURR1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3B3
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00146 WB 100 µl
VMA00146KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human COMMD1 antibody recognizes COMMD1, also known as COMM domain-containing protein 1, copper metabolism gene MURR1 and protein Murr1. COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B signaling (de Bie et al. 2005).

Mouse anti Human COMMD1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti COMMD1 detects a band of approximately 21 kDa in K562 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human COMMD1 (NP_689729) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8N668 Related reagents
Entrez Gene
COMMD1 Related reagents
GO Terms
GO:0005507 copper ion binding
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0030054 cell junction
GO:0031398 positive regulation of protein ubiquitination
GO:0031462 Cul2-RING ubiquitin ligase complex
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0042803 protein homodimerization activity
GO:0055070 copper ion homeostasis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of COMMD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Product Specific References

Further Reading

  1. de Bie P et al. (2005) The many faces of the copper metabolism protein MURR1/COMMD1.
    J Hered. 96 (7): 803-11.

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