COMMD1 antibody | OTI3B3
Mouse anti COMMD1
COMMD1 antibody recognizes the COMM domain-containing protein 1, also known as MURR1. The antibody has been extensively validated for western blotting using whole cell lysates.
COMMD1 Antibody with Control Lysate
COMMD1 antibody recognizes the COMM domain-containing protein 1, also known as MURR1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3B3
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00146 | WB | 100 µl | ||||
| VMA00146KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human COMMD1 antibody recognizes COMMD1, also known as COMM domain-containing protein 1, copper metabolism gene MURR1 and protein Murr1. COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B signaling (de Bie et al. 2005).
Mouse anti Human COMMD1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human COMMD1 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti COMMD1 detects a band of approximately 21 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human COMMD1 (NP_689729) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q8N668 Related reagents
- Entrez Gene
- COMMD1 Related reagents
- GO Terms
- GO:0005507 copper ion binding
- GO:0005737 cytoplasm
- GO:0005730 nucleolus
- GO:0030054 cell junction
- GO:0031398 positive regulation of protein ubiquitination
- GO:0031462 Cul2-RING ubiquitin ligase complex
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032434 regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0042803 protein homodimerization activity
- GO:0055070 copper ion homeostasis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of COMMD1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Product Specific References
Further Reading
-
de Bie P et al. (2005) The many faces of the copper metabolism protein MURR1/COMMD1.
J Hered. 96 (7): 803-11.
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