Collagen VII antibody | LH7.2

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Anti Human Collagen VII Antibody, clone LH7.2 gallery image 1 — Human ileum cryosection stained with Mouse anti Human Collagen VII (**MCA597GA**) follwed by Histar Detection System (STAR3000)

Mouse anti Human Collagen VII

Product Type: Monoclonal Antibody
Clone: LH7.2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA597GA	C			0.1 mg

Mouse anti Human collagen VII antibody, clone LH7.2 recognizes the carboxy-terminus of type VII collagen (Kirkham et al. 1989). Collagen VII is a basement membrane protein in stratified squamous epithelia which is involved in membrane organization through interaction with other ECM components (Gammon et al. 1992). It is composed of three identical alpha chains, each having an amino-terminal non-collagenous domain and a carboxy-terminal collagenous domain.

Mouse anti Human collagen VII, clone LH7.2 has proved effective for immunohistochemical identification of basement membrane in human skin (Watson et al. 2001).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Mouse
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Crude extract of skin

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2 myeloma cell line

Storage Information

Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q02388 Related reagents
Entrez Gene: COL7A1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0007155 cell adhesion; GO:0004867 serine-type endopeptidase inhibitor activity; GO:0005590 collagen type VII; GO:0005604 basement membrane; GO:0008544 epidermis development
Regulatory: For research purposes only

Applications of Collagen VII antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen		1/25	1/100

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Collagen VII antibody

Heagerty, A.H. et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis.
Br J Dermatol. 115 (2): 125-31.
Leigh, I.M. et al. (1987) LH7.2 Monoclonal antibody detects type VII collagen in the sublamina densa zone of ectodermally-derived epithelia, including skin.
Epithelia 1: 17-29.
Kirkham, N. et al. (1989) Type VII collagen antibody LH 7.2 identifies basement membrane characteristics of thin malignant melanomas.
J Pathol. 157 (3): 243-7.
Leigh, I.M. et al. (1988) Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
J Invest Dermatol. 90 (5): 639-42.
Ghohestani, R.F. et al. (1998) IgE antibodies in sera from patients with bullous pemphigoid are autoantibodies preferentially directed against the 230-kDa epidermal antigen (BP230).
J Clin Immunol. 18: 202-9.
Craven, N.M. et al. (1997) Clinical features of photodamaged human skin are associated with a reduction in collagen VII.
Br J Dermatol. 137: 344-50.
Al-Refu, K and Goodfield, M. (2011) Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
Clin Exp Dermatol. 36: 63-8.
Herndon, D.N. et al. (1995) Characterization of growth hormone enhanced donor site healing in patients with large cutaneous burns.
Ann Surg. 221: 649-56.
Al-Refu, K. and Goodfield, M. (2009) Basement membrane changes in lichen planopilaris.
J Eur Acad Dermatol Venereol. 23: 1289-93.
Watson, R.E. et al. (2001) A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
J Invest Dermatol. 116: 672-8.
Jolicoeur, F. et al. (2003) Basal cells of second trimester fetal breasts: immunohistochemical study of myoepithelial precursors.
Pediatr Dev Pathol. 6: 398-413.

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