Collagen VII antibody | LH7.2
Mouse anti Human collagen VII, clone LH7.2 has proved effective for immunohistochemical identification of basement membrane in human skin (Watson et al. 2001).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Mouse Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Crude extract of skin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005590 collagen type VII
- GO:0005604 basement membrane
- GO:0008544 epidermis development
- For research purposes only
Applications of Collagen VII antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/25||1/100|
Secondary Antibodies Available
Product Specific References
References for Collagen VII antibody
Heagerty, A.H. et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis.
Br J Dermatol. 115 (2): 125-31.
Leigh, I.M. et al. (1987) LH7.2 Monoclonal antibody detects type VII collagen in the sublamina densa zone of ectodermally-derived epithelia, including skin.
Epithelia 1: 17-29.
Kirkham, N. et al. (1989) Type VII collagen antibody LH 7.2 identifies basement membrane characteristics of thin malignant melanomas.
J Pathol. 157 (3): 243-7.
Leigh, I.M. et al. (1988) Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
J Invest Dermatol. 90 (5): 639-42.
Ghohestani, R.F. et al. (1998) IgE antibodies in sera from patients with bullous pemphigoid are autoantibodies preferentially directed against the 230-kDa epidermal antigen (BP230).
J Clin Immunol. 18: 202-9.
Craven, N.M. et al. (1997) Clinical features of photodamaged human skin are associated with a reduction in collagen VII.
Br J Dermatol. 137: 344-50.
Al-Refu, K and Goodfield, M. (2011) Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
Clin Exp Dermatol. 36: 63-8.
Herndon, D.N. et al. (1995) Characterization of growth hormone enhanced donor site healing in patients with large cutaneous burns.
Ann Surg. 221: 649-56.
Al-Refu, K. and Goodfield, M. (2009) Basement membrane changes in lichen planopilaris.
J Eur Acad Dermatol Venereol. 23: 1289-93.
Watson, R.E. et al. (2001) A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
J Invest Dermatol. 116: 672-8.
Jolicoeur, F. et al. (2003) Basal cells of second trimester fetal breasts: immunohistochemical study of myoepithelial precursors.
Pediatr Dev Pathol. 6: 398-413.
Fluorescent SpectraviewerWatch the Tool Tutorial Video ▸
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra