Collagen VII antibody | LH7.2
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C ResetMouse anti Human Collagen VII
- Product Type
- Monoclonal Antibody
- Clone
- LH7.2
- Isotype
- IgG1
- Specificity
- Collagen VII
Antibody Quality and Performance Guarantee
| Mouse anti Human collagen VII antibody, clone LH7.2 recognizes the carboxy-terminus of type VII collagen (Kirkham et al. 1989). Collagen VII is a basement membrane protein in stratified squamous epithelia which is involved in membrane organization through interaction with other ECM components (Gammon et al. 1992). Collagen VII is composed of three identical alpha chains, each having an amino-terminal non-collagenous domain and a carboxy-terminal collagenous domain. Mouse anti Human collagen VII, clone LH7.2 has proved effective for immunohistochemical identification of basement membrane in human skin (Watson et al. 2001). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Crude extract of skin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
1/25 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Collagen VII antibody
-
Heagerty, A.H. et al. (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis.
Br J Dermatol. 115 (2): 125-31. -
Leigh, I.M. et al. (1987) LH7.2 Monoclonal antibody detects type VII collagen in the sublamina densa zone of ectodermally-derived epithelia, including skin.
Epithelia 1: 17-29. -
Leigh, I.M. et al. (1988) Type VII collagen is a normal component of epidermal basement membrane, which shows altered expression in recessive dystrophic epidermolysis bullosa.
J Invest Dermatol. 90 (5): 639-42. -
Kirkham, N. et al. (1989) Type VII collagen antibody LH 7.2 identifies basement membrane characteristics of thin malignant melanomas.
J Pathol. 157 (3): 243-7. -
Herndon, D.N. et al. (1995) Characterization of growth hormone enhanced donor site healing in patients with large cutaneous burns.
Ann Surg. 221: 649-56. -
Craven, N.M. et al. (1997) Clinical features of photodamaged human skin are associated with a reduction in collagen VII.
Br J Dermatol. 137: 344-50. -
Ghohestani, R.F. et al. (1998) IgE antibodies in sera from patients with bullous pemphigoid are autoantibodies preferentially directed against the 230-kDa epidermal antigen (BP230).
J Clin Immunol. 18: 202-9. -
Watson, R.E. et al. (2001) A short-term screening protocol, using fibrillin-1 as a reporter molecule, for photoaging repair agents.
J Invest Dermatol. 116: 672-8.
View The Latest Product References
-
Jolicoeur, F. et al. (2003) Basal cells of second trimester fetal breasts: immunohistochemical study of myoepithelial precursors.
Pediatr Dev Pathol. 6: 398-413. -
Al-Refu, K. and Goodfield, M. (2009) Basement membrane changes in lichen planopilaris.
J Eur Acad Dermatol Venereol. 23: 1289-93. -
Al-Refu, K. and Goodfield, M. (2011) Immunohistochemistry of ultrastructural changes in scarring lupus erythematosus.
Clin Exp Dermatol. 36: 63-8.
- UniProt
- Q02388
- Entrez Gene
- COL7A1
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005590 collagen type VII
- GO:0005604 basement membrane
- GO:0008544 epidermis development
MCA597GA
0814
166954
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