Collagen IV antibody | NLI/53
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Mouse anti Human collagen IV antibody, clone NLI/53 recognizes human collagen IV.
Product Details
- Target Species
- Human
- Product Form
- Purified IgM - lyophilised
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Preparation
- Purified IgM prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Mannitol
0.1% Dextran - Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P02462 Related reagents
- P08572 Related reagents
- Q01955 Related reagents
- P53420 Related reagents
- P29400 Related reagents
- Q14031 Related reagents
- Entrez Gene
- COL4A1 Related reagents
- COL4A2 Related reagents
- COL4A5 Related reagents
- COL4A4 Related reagents
- COL4A3 Related reagents
- COL4A6 Related reagents
- GO Terms
- GO:0001525 angiogenesis
- GO:0005201 extracellular matrix structural constituent
- GO:0007411 axon guidance
- GO:0048407 platelet-derived growth factor binding
- GO:0005515 protein binding
- GO:0005587 collagen type IV
- GO:0016525 negative regulation of angiogenesis
- GO:0030198 extracellular matrix organization
- GO:0005605 basal lamina
- GO:0032836 glomerular basement membrane development
- Regulatory
- For research purposes only
Applications of Collagen IV antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/800 | 1/1200 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/5 | 1/50 | |
| Immunohistology - Paraffin | 1/5 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | ||
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C E P WB | ||
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | ||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C E WB | ||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E |
Product Specific References
References for Collagen IV antibody
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Cooper, L.J. et al. (2005) An investigation into the composition of amniotic membrane used for ocular surface reconstruction
Cornea. 24: 722-9.
