Collagen IV antibody | NLI/53

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Mouse anti Human Collagen IV

Product Type
Monoclonal Antibody
Clone
NLI/53
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0121 C E IF P 1 ml
Mouse anti Human collagen IV antibody, clone NLI/53 recognizes human collagen IV.

Product Details

Target Species
Human
Product Form
Purified IgM - lyophilised
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgM prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Mannitol
0.1% Dextran
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P02462 Related reagents
P08572 Related reagents
Q01955 Related reagents
P53420 Related reagents
P29400 Related reagents
Q14031 Related reagents
Entrez Gene
COL4A1 Related reagents
COL4A2 Related reagents
COL4A5 Related reagents
COL4A4 Related reagents
COL4A3 Related reagents
COL4A6 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
Regulatory
For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/800 1/1200
Immunofluorescence
Immunohistology - Frozen 1/5 1/50
Immunohistology - Paraffin 1/5 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for Collagen IV antibody

  1. Cooper, L.J. et al. (2005) An investigation into the composition of amniotic membrane used for ocular surface reconstruction
    Cornea. 24: 722-9.