Collagen IV antibody | NLI/53

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Mouse anti Human Collagen IV

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
1 ml loader

Mouse anti Human collagen IV antibody, clone NLI/53 recognizes human collagen IV.

Product Details

Target Species
Product Form
Purified IgM - lyophilised
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Purified IgM prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1% Mannitol
1% Dextran
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml after reconstitution

Storage Information

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guaranteed until date of expiry. Please see product label.

More Information

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Entrez Gene
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COL4A2 Related reagents
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GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development
For research purposes only

Applications of Collagen IV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/800 1/1200
Immunohistology - Frozen 1/5 1/50
Immunohistology - Paraffin 1/5 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Product Specific References

References for Collagen IV antibody

  1. Cooper, L.J. et al. (2005) An investigation into the composition of amniotic membrane used for ocular surface reconstruction
    Cornea. 24: 722-9.