Mouse anti Human collagen IV antibody, clone NLI/53 recognizes human collagen IV.
- Target Species
- Product Form
- Purified IgM - lyophilised
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Purified IgM prepared by ion exchange chromatography from ascites
- Buffer Solution
- 0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
- Preservative Stabilisers
- 1% Mannitol
- Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml after reconstitution
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
extracellular matrix structural constituent
platelet-derived growth factor binding
collagen type IV
negative regulation of angiogenesis
extracellular matrix organization
glomerular basement membrane development
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Collagen IV antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Collagen IV antibody
Cooper, L.J. et al. (2005) An investigation into the composition of amniotic membrane used for ocular surface reconstruction
Cornea. 24: 722-9.
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