Collagen III antibody | NLI/42
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Mouse anti Human collagen III antibody, clone NLI/42 recognizes human collagen type III encoded by the COL3A1 gene. Collagen type III is expressed in most soft connective tissues.
Product Details
- Target Species
- Human
- Product Form
- Purified IgM - lyophilised
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgM prepared by ion exchange chromatography from ascites
- Buffer Solution
- 0.15M Sodium Chloride
0.01M Sodium Phosphate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Approx. Protein Concentrations
- 0.1mg/ml after reconstitution.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of Collagen III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/2000 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/20 | 1/80 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
|
|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
|
|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
|
|
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
|
Product Specific References
References for Collagen III antibody
-
Ashjian, P. et al. (2004) Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy.
Tissue Eng. 10 (3-4): 411-20. -
Gonzalez, G. et al. (2007) Methods and Therapies for Treating Inflammatory Conditions with Exposed Collagen
US Patent Application US20070071676 A1
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