Collagen III antibody | NLI/42

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Mouse anti Human Collagen III

Product Type
Monoclonal Antibody
Clone
NLI/42
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0081 C E IF 1 ml
Mouse anti Human collagen III antibody, clone NLI/42 recognizes human collagen type III encoded by the COL3A1 gene. Collagen type III is expressed in most soft connective tissues.

Product Details

Target Species
Human
Product Form
Purified IgM - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgM prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate/Borate buffered saline
Preservative Stabilisers
1% Mannitol
1% Dextran
Approx. Protein Concentrations
0.1mg/ml after reconstitution.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P02461 Related reagents
Entrez Gene
COL3A1 Related reagents
Regulatory
For research purposes only

Applications of Collagen III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/2000
Immunofluorescence
Immunohistology - Frozen 1/20 1/80
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Product Specific References

References for Collagen III antibody

  1. Ashjian, P. et al. (2004) Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy.
    Tissue Eng. 10 (3-4): 411-20.
  2. Gonzalez, G. et al. (2007) Methods and Therapies for Treating Inflammatory Conditions with Exposed Collagen
    US Patent Application US20070071676 A1