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Collagen II antibody | COLL-II

Mouse anti Human Collagen II

Product Type
Monoclonal Antibody
Collagen II

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SDS Safety Datasheet SDS
E IF * P WB 0.1 ml loader
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Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites - liquid
Preservative Stabilisers
None present
Human (cartilage specific) CNBr-cleaved collagen 2.
For research purposes only
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunofluorescence 1 Neat 1/5
Immunohistology - Paraffin 1/50
Western Blotting 1/1000 1/2000
  1. 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Collagen II antibody

  1. Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
    Histochemistry. 82 (2): 149-58.
  2. Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
    Br J Haematol. 90 (4): 757-66.
  3. Herrero-Mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
    J Tissue Eng Regen Med. 11 (4): 989-1001.

Further Reading

  1. Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
    Biochemistry. 11 (26): 4903-9.

Entrez Gene


151166 154869 158986 160136 165355 165846

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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