Collagen II antibody | COLL-II


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P RE ResetMouse anti Human Collagen II
- Product Type
- Monoclonal Antibody
- Clone
- COLL-II
- Isotype
- IgG1
- Specificity
- Collagen II
Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine Sheep Mouse Dog Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ascites - liquid
- Preservative Stabilisers
- None present
- Immunogen
- Human (cartilage specific) CNBr-cleaved collagen 2.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/100 | 1/500 |
Immunofluorescence 1 | ![]() |
Neat | 1/5 |
Immunohistology - Paraffin | ![]() |
1/50 | |
Western Blotting | ![]() |
1/1000 | 1/2000 |
- 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
References for Collagen II antibody
-
Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
Histochemistry. 82 (2): 149-58. -
Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
Br J Haematol. 90 (4): 757-66. -
Herrero-Mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
J Tissue Eng Regen Med. 11 (4): 989-1001.
Further Reading
-
Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Biochemistry. 11 (26): 4903-9.
- RRID
- AB_2082176
- UniProt
- P02458
- Entrez Gene
- COL2A1
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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