Collagen II antibody | COLL-II
Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine Sheep Mouse Dog Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ascites - liquid
- Preservative Stabilisers
- None present
- Immunogen
- Human (cartilage specific) CNBr-cleaved collagen 2.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of Collagen II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/500 | |
| Immunofluorescence 1 | Neat | 1/5 | |
| Immunohistology - Paraffin | 1/50 | ||
| Western Blotting | 1/1000 | 1/2000 |
- 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Product Specific References
References for Collagen II antibody
-
Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
Br J Haematol. 90 (4): 757-66. -
Herrero-mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
J Tissue Eng Regen Med. 11 (4): 989-1001. -
Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
Histochemistry. 82 (2): 149-58. -
Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Iranian J basic Med Res. 11: 10-17
Further Reading
-
Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Biochemistry. 11 (26): 4903-9.
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