Collagen II antibody | COLL-II

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Anti Human Collagen II Antibody, clone COLL-II thumbnail image 1

Anti Human Collagen II Antibody, clone COLL-II thumbnail image 2

Anti Human Collagen II Antibody, clone COLL-II gallery image 1 — FFPE human colon stained with Mouse Anti-Human Collagen II (**2150-0040**), biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.

Anti Human Collagen II Antibody, clone COLL-II gallery image 2 — FFPE human placenta stained with Mouse Anti-Human Collagen II (**2150-0040**), biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.

Mouse anti Human Collagen II

Product Type: Monoclonal Antibody
Clone: COLL-II
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
2150-0040	E IF* P WB			0.1 ml

Summary
References (5)

Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Bovine
Sheep
Mouse
Dog
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Ascites - liquid

Preservative Stabilisers

None present

Immunogen

Human (cartilage specific) CNBr-cleaved collagen 2.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P02458 Related reagents
Entrez Gene: COL2A1 Related reagents
Regulatory: For research purposes only

Applications of Collagen II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/100	1/500
Immunofluorescence ¹	Neat	1/5
Immunohistology - Paraffin		1/50
Western Blotting	1/1000	1/2000

¹Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Collagen II antibody

Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
Br J Haematol. 90 (4): 757-66.
Herrero-mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
J Tissue Eng Regen Med. 11 (4): 989-1001.
Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
Histochemistry. 82 (2): 149-58.
Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Iranian J basic Med Res. 11: 10-17