Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ascites - liquid
- Preservative Stabilisers
- None present
- Human (cartilage specific) CNBr-cleaved collagen 2.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Collagen II antibody
|Immunohistology - Paraffin
- 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Collagen II antibody
Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
Br J Haematol. 90 (4): 757-66.
Herrero-mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
J Tissue Eng Regen Med. 11 (4): 989-1001.
Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
Histochemistry. 82 (2): 149-58.
Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Iranian J basic Med Res. 11: 10-17
Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Biochemistry. 11 (26): 4903-9.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra