Collagen II antibody | COLL-II
Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Bovine Sheep Mouse Dog Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ascites - liquid
- Preservative Stabilisers
- None present
- Human (cartilage specific) CNBr-cleaved collagen 2.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P02458 Related reagents
- Entrez Gene
- COL2A1 Related reagents
- For research purposes only
Applications of Collagen II antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin||1/50|
- 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Collagen II antibody
Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
Br J Haematol. 90 (4): 757-66.
Herrero-mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
J Tissue Eng Regen Med. 11 (4): 989-1001.
Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
Histochemistry. 82 (2): 149-58.
Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Iranian J basic Med Res. 11: 10-17
Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Biochemistry. 11 (26): 4903-9.