Collagen II antibody | COLL-II

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Mouse anti Human Collagen II

Product Type
Monoclonal Antibody
Clone
COLL-II
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0040 E IF* P WB 0.1 ml
Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Bovine
Sheep
Mouse
Dog
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ascites - liquid
Preservative Stabilisers
None present
Immunogen
Human (cartilage specific) CNBr-cleaved collagen 2.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02458 Related reagents
Entrez Gene
COL2A1 Related reagents
Regulatory
For research purposes only

Applications of Collagen II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunofluorescence 1 Neat 1/5
Immunohistology - Paraffin 1/50
Western Blotting 1/1000 1/2000
  1. 1Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Collagen II antibody

  1. Wilkins, B.S. & Jones, D.B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow.
    Br J Haematol. 90 (4): 757-66.
  2. Herrero-mendez, A. et al. (2017) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.
    J Tissue Eng Regen Med. 11 (4): 989-1001.
  3. Glant, T.T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II.
    Histochemistry. 82 (2): 149-58.
  4. Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
    Iranian J basic Med Res. 11: 10-17

Further Reading

  1. Miller, E.J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
    Biochemistry. 11 (26): 4903-9.