Collagen I antibody | NFI/20

Mouse anti Human Collagen I

Product Type
Monoclonal Antibody
Clone
NFI/20
Isotype
IgG1
Specificity
Collagen I

Product Code Applications Pack Size List Price Your Price Qty
2150-0001
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Mouse anti Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite. Mouse anti human Collagen I, clone NFI/20 does not cross-react with collagen types II, III and IV. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.

Mutations in the encoding gene are associated with osteogenesis imperfecta (Peng et al. 2012), Ehlers Danlos syndrome (Nuytinck et al. 2000) and idiopathic juvenile osteoporosis (Pocock et al. 1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihme et al. 1984),

Target Species
Human
Product Form
Ig fraction - lyophilized
Reconstitution
Use 1 ml of sterile distilled water.
Preparation
Ig fraction prepared by ammonium sulphate precipitation and DEAE-cellulose chromatography from ascites
Buffer Solution
0.1M Sodium Chloride
0.01M Sodium Phosphate
0.01M Sodium Borate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Human collagen type I from placenta.
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Immunohistology - Frozen 1/20
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Collagen I antibody

  1. Zorin, V. et al. (2015) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
    J Tissue Eng Regen Med. 11 (3): 778-86.
  2. Zorin, V.L. et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
    Biomed Mater. 9 (5): 055005.
  3. Seedhom, B.B. et al. (2007) In-situ engineering of cartilage repair: a pre-clinical in-vivo exploration of a novel system.
    Proc Inst Mech Eng H. 221: 475-88.

RRID
AB_620102
UniProt
P02452
P08123
Entrez Gene
COL1A1
COL1A2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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