Collagen I antibody | NFI/20
No associated image currently available
Mouse anti Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite. Mouse anti human Collagen I, clone NFI/20 does not cross-react with collagen types II, III and IV. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Mutations in the encoding gene are associated with osteogenesis imperfecta (Peng et al. 2012), Ehlers Danlos syndrome (Nuytinck et al. 2000) and idiopathic juvenile osteoporosis (Pocock et al. 1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihme et al. 1984),
Mutations in the encoding gene are associated with osteogenesis imperfecta (Peng et al. 2012), Ehlers Danlos syndrome (Nuytinck et al. 2000) and idiopathic juvenile osteoporosis (Pocock et al. 1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihme et al. 1984),
Product Details
- Target Species
- Human
- Product Form
- Ig fraction - lyophilised
- Reconstitution
- Use 1 ml of sterile distilled water.
- Preparation
- Ig fraction prepared by ammonium sulphate precipitation and DEAE-cellulose chromatography
- Preservative Stabilisers
- Contains mannitol, dextran & salts
- Immunogen
- Human collagen type I from placenta.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
Applications of Collagen I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | ||
| Immunohistology - Frozen | 1/20 | ||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Collagen I antibody
-
Zorin, V. et al. (2015) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
J Tissue Eng Regen Med. 11 (3): 778-86. -
Zorin, V.L. et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
Biomed Mater. 9 (5): 055005. -
Seedhom, B.B. et al. (2007) In-situ engineering of cartilage repair: a pre-clinical in-vivo exploration of a novel system.
Proc Inst Mech Eng H. 221: 475-88.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
