Clathrin Heavy Chain antibody

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Anti Clathrin Heavy Chain Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human clathrin heavy chain antibody (**VMA00411**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 4 second exposure. Arrow points to clathrin heavy chain (molecular weight 192 kDa).

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Mouse anti Clathrin Heavy Chain

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Clathrin Heavy Chain

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VMA00411 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human clathrin heavy chain antibody recognizes clathrin heavy chain , also known as CLTC, CHC, clathrin heavy chain on chromosome 17 (CHC17) and CLTCL2.

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains (provided by RefSeq, Jul 2008).

Mouse anti Human clathrin heavy chain antibody detects a band of 192 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti clathrin heavy chain detects a band of approximately 192 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E.coli-derived recombinant protein corresponding to aa 1521-1675 of human clathrin heavy chain
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q00610
Entrez Gene: CLTC
GO Terms: GO:0005515 protein binding; GO:0005829 cytosol; GO:0005198 structural molecule activity; GO:0005819 spindle; GO:0006886 intracellular protein transport; GO:0006892 post-Golgi vesicle-mediated transport; GO:0007067 mitosis; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007411 axon guidance; View More GO Terms; GO:0030130 clathrin coat of trans-Golgi network vesicle; GO:0030132 clathrin coat of coated pit; GO:0031623 receptor internalization; GO:0033572 transferrin transport; GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway; GO:0042470 melanosome; GO:0048011 nerve growth factor receptor signaling pathway