Chk1 antibody | 2G1D5

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Mouse anti Chk1

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6041 WB 50 µg
Mouse anti Chk1 antibody, clone 2G1D5 recognizes Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1.

Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003).

Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
Recombinant human Chk1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

O14757 Related reagents
Entrez Gene
CHEK1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000077 DNA damage checkpoint
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0005515 protein binding
GO:0000794 condensed nuclear chromosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005815 microtubule organizing center
GO:0006281 DNA repair
GO:0006468 protein phosphorylation
GO:0007131 reciprocal meiotic recombination
GO:0007276 gamete generation
GO:0008285 negative regulation of cell proliferation
GO:0090399 replicative senescence
For research purposes only

Applications of Chk1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse ant Chk1 recognizes a band of ~55 kDa by western blot
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