Chk1 antibody | 2G1D5
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WB ResetMouse anti Chk1
- Product Type
- Monoclonal Antibody
- Clone
- 2G1D5
- Isotype
- IgG1
- Specificity
- Chk1
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Antibody Quality and 
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| Mouse anti Chk1 antibody, clone 2G1D5 recognizes Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1. Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003). Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Recombinant human Chk1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse ant Chk1 recognizes a band of ~55 kDa by western blot
- Synonyms
- Checkpoint KINASE-1
- UniProt
- O14757
- Entrez Gene
- CHEK1
- GO Terms
- GO:0006260 DNA replication
- GO:0000077 DNA damage checkpoint
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0005515 protein binding
- GO:0000794 condensed nuclear chromosome
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005815 microtubule organizing center
- View More GO Terms
- GO:0006281 DNA repair
- GO:0006468 protein phosphorylation
- GO:0007131 reciprocal meiotic recombination
- GO:0007276 gamete generation
- GO:0008285 negative regulation of cell proliferation
- GO:0090399 replicative senescence
View more products with CHK1 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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