Chk1 antibody | 2G1D5

Mouse anti Chk1

Product Type
Monoclonal Antibody
Clone
2G1D5
Isotype
IgG1
Specificity
Chk1

Product Code Applications Pack Size List Price Your Price Qty
MCA6041
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Chk1 antibody, clone 2G1D5 recognizes Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1.

Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003).

Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Immunogen
Recombinant human Chk1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse ant Chk1 recognizes a band of ~55 kDa by western blot

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Synonyms
Checkpoint KINASE-1
UniProt
O14757
Entrez Gene
CHEK1
GO Terms
GO:0006260 DNA replication
GO:0000077 DNA damage checkpoint
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0005515 protein binding
GO:0000794 condensed nuclear chromosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005815 microtubule organizing center
GO:0006281 DNA repair
GO:0006468 protein phosphorylation
GO:0007131 reciprocal meiotic recombination
GO:0007276 gamete generation
GO:0008285 negative regulation of cell proliferation
GO:0090399 replicative senescence
View more products with CHK1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products