CHI3L1 antibody | C02-4H12

100% Secure

Mouse anti Human CHI3L1

Product Type
Monoclonal Antibody
Clone
C02-4H12
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5962GA E 0.1 mg
Mouse anti Human CHI3L1, clone C02-4H12, recognizes human CHI3L1 (also known as HCgp39 & YKL-40). CHI3L1 is a secreted glcoprotein belonging to the family of chitinase-like lectins but lacking enzymatic properties.

CHI3L1 is produced by smooth muscle cells, macrophages, neutrophils, chondrocytes and osteosarcoma cels. While the general mode of action remains to determined, CHI3L1 has been implicated in angiogenesis via VEGF signalling (Riabov et al. 2014). CHI3L1 has aslo emerged as a biomarker of many chronic inflammatory conditions such as Alzheimer`s disease (Craig-Schapiro et al. 2010), rheumatoid arthritis (Lee et al. 2011) and multiple sclerosis (Comabella et al. 2010).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to aa 22-383 of human CHI3L1/YKL-40
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mouse were fused with cells of the mouse myeloma SP2/0 cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P36222 Related reagents
Entrez Gene
CHI3L1 Related reagents
GO Terms
GO:0004568 chitinase activity
GO:0005201 extracellular matrix structural constituent
GO:0005529 sugar binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006032 chitin catabolic process
GO:0043169 cation binding
Regulatory
For research purposes only

Applications of CHI3L1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. Comabella, M. et al. (2010) Cerebrospinal fluid chitinase 3-like 1 levels are associated with conversion to multiple sclerosis
    Brain 133:1082-93.
  2. Craig-Schapiro, R. et al. (2010) YKL-40: a novel prognostic fluid biomarker for preclinical Alzheimer's disease
    Biol Psychiatry. 68: 903-12.
  3. Lee, C.G. et al. (2011) Role of Chitin and Chitinase/Chitinase-Like Proteins in Inflammation, Tissue Remodeling, and Injury
    Annual Review of Physiology 73: 479-501.
  4. Riabov, V. et al. (2014) Role of tumor associated macrophages in tumor angiogenesis and lymphangiogenesis
    Front Physiol. 5: 75.