CHD3 antibody

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Mouse anti CHD3

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00693
Datasheet
WB
SDS
100 µl loader

Mouse anti Human CHD3 antibody recognizes the chromodomain-helicase-DNA-binding protein 3, also known as ATP-dependent helicase CHD3 and ZFH.

CHD3 encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described (provided by RefSeq, Jul 2008).

Mouse anti Human CHD3 antibody detects a band of 260 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti CHD3 detects a band of approximately 260 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on immunogen from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of the C-terminal region of human CHD3

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q12873
Entrez Gene
CHD3
GO Terms
GO:0005515 protein binding
GO:0000785 chromatin
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0006333 chromatin assembly or disassembly
GO:0004003 ATP-dependent DNA helicase activity
GO:0005524 ATP binding
GO:0006350 transcription
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0016581 NuRD complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CHD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml