CDw210a antibody | 3F9
Rat anti Human CDw210a, clone 3F9 recognizes human CDw210a, a 90-110kD glycoprotein, also known as Interleukin-10 receptor 1 (IL-10R1).
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Recent work has reported an increase in intracellular expression of CDw210a during dendritic cell maturation, but a reduction in surface CDw210a expression, see Corinti. S. et al. for details.
This clone is reported to function as a blocking antibody. Bio-Rad recommend the use of MCA2255XZ for functional studies, see Liu. Y. et al. for details.
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Recent work has reported an increase in intracellular expression of CDw210a during dendritic cell maturation, but a reduction in surface CDw210a expression, see Corinti. S. et al. for details.
This clone is reported to function as a blocking antibody. Bio-Rad recommend the use of MCA2255XZ for functional studies, see Liu. Y. et al. for details.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Soluble human CDw210a
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q13651 Related reagents
- Entrez Gene
- IL10RA Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0004920 interleukin-10 receptor activity
- Regulatory
- For research purposes only
Applications of CDw210a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/100 | 1/200 | |
| Functional Assays | |||
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Flow Cytometry | Neat |
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:RPE | MCA6005PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CDw210a antibody
-
Liu, Y. et al. (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.
J Immunol. 158 (2): 604-13. -
O7#39;Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
EMBO J. 17 (4): 1006-18. -
Corinti, S. et al. (2001) Regulatory activity of autocrine IL-10 on dendritic cell functions.
J Immunol. 166 (7): 4312-8. -
Elbim, C. et al. (2001) Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
J Immunol. 166 (8): 5201-7. -
Fayette, J. et al. (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.
J Exp Med. 185 (11): 1909-18. -
Goodier, M.R. & Londei, M. (2000) Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
J Immunol. 165 (1): 139-47. -
Levings, M.K. et al. (2001) IFN-alpha and IL-10 induce the differentiation of human type 1 T regulatory cells.
J Immunol. 166 (9): 5530-9. -
Hamel, Y. et al. (2016) A unique CD8(+) T lymphocyte signature in pediatric type 1 diabetes.
J Autoimmun. 73: 54-63.
