CDw210a antibody | 3F9

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Anti Human CDw210a Antibody, clone 3F9 thumbnail image 1

Anti Human CDw210a Antibody, clone 3F9 thumbnail image 2

Anti Human CDw210a Antibody, clone 3F9 gallery image 1 — Staining of human peripheral blood lymphocytes with biotinylated Rat anti Human CDw210a antibody, clone 3F9 (**MCA2255B**).

Anti Human CDw210a Antibody, clone 3F9 gallery image 2 — Staining of human peripheral blood lymphocytes with RPE conjugated Rat anti Human CDw210aantibody, clone 3F9 (**MCA2255PE**).

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Rat anti Human CDw210a

Product Type: Monoclonal Antibody
Clone: 3F9
Isotype: IgG2a
Specificity: CDw210a

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Secondary Antibodies
References (7)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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MCA2255GA Datasheet Datasheet SDS SDS	F *	0.1 mg		Log in	Get Quote
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Rat anti Human CDw210a, clone 3F9 recognizes human CDw210a, a 90-110 kDa glycoprotein, also known as Interleukin-10 receptor 1 (IL-10R1).
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Intracellular expression of CDw210a increases during dendritic cell maturation, while surface CDw210a expression decreases (Corinti et al. 2001) .
Rat anti Human CDw210a, clone 3F9 functions as a blocking antibody(Liu et al. 1997).

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
Carrier Free: Yes
Immunogen: Soluble human CDw210a
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		1/10	1/50

¹Due to low expression of CDw210a, sensitive staining techniques may be required.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

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References for CDw210a antibody

Liu, Y. et al. (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.
J Immunol. 158 (2): 604-13.
O7#39;Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
EMBO J. 17 (4): 1006-18.
Elbim, C. et al. (2001) Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
J Immunol. 166 (8): 5201-7.
Fayette, J. et al. (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.
J Exp Med. 185 (11): 1909-18.
Goodier, M.R. & Londei, M. (2000) Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
J Immunol. 165 (1): 139-47.
Levings, M.K. et al. (2001) IFN-alpha and IL-10 induce the differentiation of human type 1 T regulatory cells.
J Immunol. 166 (9): 5530-9.
Hamel, Y. et al. (2016) A unique CD8(+) T lymphocyte signature in pediatric type 1 diabetes.
J Autoimmun. 73: 54-63.

Synonyms: IL-10R1
RRID: AB_2125488
UniProt: Q13651
Entrez Gene: IL10RA
GO Terms: GO:0005886 plasma membrane; GO:0007596 blood coagulation; GO:0016021 integral to membrane; GO:0004920 interleukin-10 receptor activity