CDw210a antibody | 3F9
Rat anti Human CDw210a, clone 3F9 recognizes human CDw210a, a 90-110 kDa glycoprotein, also known as Interleukin-10 receptor 1 (IL-10R1).
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Intracellular expression of CDw210a increases during dendritic cell maturation, while surface CDw210a expression decreases (Corinti et al. 2001) .
Rat anti Human CDw210a, clone 3F9 functions as a blocking antibody(Liu et al. 1997).
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Intracellular expression of CDw210a increases during dendritic cell maturation, while surface CDw210a expression decreases (Corinti et al. 2001) .
Rat anti Human CDw210a, clone 3F9 functions as a blocking antibody(Liu et al. 1997).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Soluble human CDw210a
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13651
- Entrez Gene
- IL10RA
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0004920 interleukin-10 receptor activity
- Regulatory
- For research purposes only
Applications of CDw210a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Flow Cytometry | Neat |
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:RPE | MCA6005PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CDw210a antibody
-
Liu, Y. et al. (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.
J Immunol. 158 (2): 604-13. -
O7#39;Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
EMBO J. 17 (4): 1006-18. -
Elbim, C. et al. (2001) Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
J Immunol. 166 (8): 5201-7. -
Fayette, J. et al. (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.
J Exp Med. 185 (11): 1909-18. -
Goodier, M.R. & Londei, M. (2000) Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
J Immunol. 165 (1): 139-47. -
Levings, M.K. et al. (2001) IFN-alpha and IL-10 induce the differentiation of human type 1 T regulatory cells.
J Immunol. 166 (9): 5530-9. -
Hamel, Y. et al. (2016) A unique CD8(+) T lymphocyte signature in pediatric type 1 diabetes.
J Autoimmun. 73: 54-63.
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