CDw210a antibody | 3F9
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F ResetRat anti Human CDw210a
- Product Type
- Monoclonal Antibody
- Clone
- 3F9
- Isotype
- IgG2a
- Specificity
- CDw210a
Antibody Quality and 
Unrivaled 
| Rat anti Human CDw210a, clone 3F9 recognizes human CDw210a, a 90-110 kDa glycoprotein, also known as Interleukin-10 receptor 1 (IL-10R1). CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low. Intracellular expression of CDw210a increases during dendritic cell maturation, while surface CDw210a expression decreases (Corinti et al. 2001) . Rat anti Human CDw210a, clone 3F9 functions as a blocking antibody(Liu et al. 1997). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Soluble human CDw210a
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/10 | 1/50 |
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CDw210a antibody
-
Liu, Y. et al. (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.
J Immunol. 158 (2): 604-13. -
O7#39;Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
EMBO J. 17 (4): 1006-18. -
Elbim, C. et al. (2001) Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
J Immunol. 166 (8): 5201-7. -
Fayette, J. et al. (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.
J Exp Med. 185 (11): 1909-18. -
Goodier, M.R. & Londei, M. (2000) Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
J Immunol. 165 (1): 139-47. -
Levings, M.K. et al. (2001) IFN-alpha and IL-10 induce the differentiation of human type 1 T regulatory cells.
J Immunol. 166 (9): 5530-9. -
Hamel, Y. et al. (2016) A unique CD8(+) T lymphocyte signature in pediatric type 1 diabetes.
J Autoimmun. 73: 54-63.
- Synonyms
- IL-10R1
- RRID
- AB_2125488
- UniProt
- Q13651
- Entrez Gene
- IL10RA
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0004920 interleukin-10 receptor activity
View more products with CDw210a specificity
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