CDw210a antibody | 3F9

Rat anti Human CDw210a

Product Type
Monoclonal Antibody
Clone
3F9
Isotype
IgG2a
Specificity
CDw210a

Product Code Applications Pack Size List Price Your Price Qty
MCA2255GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Human CDw210a, clone 3F9 recognizes human CDw210a, a 90-110 kDa glycoprotein, also known as Interleukin-10 receptor 1 (IL-10R1).
CDw210a is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low.
Intracellular expression of CDw210a increases during dendritic cell maturation, while surface CDw210a expression decreases (Corinti et al. 2001) .
Rat anti Human CDw210a, clone 3F9 functions as a blocking antibody(Liu et al. 1997).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Soluble human CDw210a
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/50
  1. 1Due to low expression of CDw210a, sensitive staining techniques may be required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CDw210a antibody

  1. Liu, Y. et al. (1997) The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.
    J Immunol. 158 (2): 604-13.
  2. O7#39;Farrell, A.M. et al. (1998) IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways.
    EMBO J. 17 (4): 1006-18.
  3. Elbim, C. et al. (2001) Intracellular pool of IL-10 receptors in specific granules of human neutrophils: differential mobilization by proinflammatory mediators.
    J Immunol. 166 (8): 5201-7.
  4. Fayette, J. et al. (1997) Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.
    J Exp Med. 185 (11): 1909-18.
  5. Goodier, M.R. & Londei, M. (2000) Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
    J Immunol. 165 (1): 139-47.
  6. Levings, M.K. et al. (2001) IFN-alpha and IL-10 induce the differentiation of human type 1 T regulatory cells.
    J Immunol. 166 (9): 5530-9.
  7. Hamel, Y. et al. (2016) A unique CD8(+) T lymphocyte signature in pediatric type 1 diabetes.
    J Autoimmun. 73: 54-63.

Synonyms
IL-10R1
RRID
AB_2125488
UniProt
Q13651
Entrez Gene
IL10RA
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0004920 interleukin-10 receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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