CDK7 antibody

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Mouse anti CDK7

Cdk7 antibody recognizes cyclin-dependent kinase 7, also known as 39 kDa protein kinase, p39 Mo15, serine/threonine-protein kinase 1 or TFIIH basal transcription factor complex kinase subunit. The antibody has been extensively validated for western blotting using whole cell lysates.

CDK7 Antibody with Control Lysate

Cdk7 antibody recognizes cyclin-dependent kinase 7, also known as 39 kDa protein kinase, p39 Mo15, CDK-activating kinase 1, cell division protein kinase 7, serine/threonine-protein kinase 1 or TFIIH basal transcription factor complex kinase subunit.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00023 WB 100 µl
VMA00023KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cdk7 antibody recognizes human cyclin-dependent kinase 7, also known as 39 kDa protein kinase, cell division protein kinase 7, serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit.

Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).

Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fragment of cdk7 corresponding to a 221 amino acid sequence from the C-terminus
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P50613 Related reagents
Entrez Gene
CDK7 Related reagents
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003713 transcription coactivator activity
GO:0005739 mitochondrion
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005675 holo TFIIH complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006362 transcription elongation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0006468 protein phosphorylation
GO:0008022 protein C-terminus binding
GO:0008094 DNA-dependent ATPase activity
GO:0008283 cell proliferation
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0016032 viral reproduction
GO:0030521 androgen receptor signaling pathway
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050434 positive regulation of viral transcription
GO:0050681 androgen receptor binding
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CDK7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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