CDK7 antibody
Mouse anti CDK7
Cdk7 antibody recognizes cyclin-dependent kinase 7, also known as 39 kDa protein kinase, p39 Mo15, serine/threonine-protein kinase 1 or TFIIH basal transcription factor complex kinase subunit. The antibody has been extensively validated for western blotting using whole cell lysates.
CDK7 Antibody with Control Lysate
Cdk7 antibody recognizes cyclin-dependent kinase 7, also known as 39 kDa protein kinase, p39 Mo15, CDK-activating kinase 1, cell division protein kinase 7, serine/threonine-protein kinase 1 or TFIIH basal transcription factor complex kinase subunit.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2b
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00023 | WB | 100 µl | ||||
| VMA00023KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human cdk7 antibody recognizes human cyclin-dependent kinase 7, also known as 39 kDa protein kinase, cell division protein kinase 7, serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit.
Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).
Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.
Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).
Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fragment of cdk7 corresponding to a 221 amino acid sequence from the C-terminus
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P50613 Related reagents
- Entrez Gene
- CDK7 Related reagents
- GO Terms
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000718 nucleotide-excision repair, DNA damage removal
- GO:0003713 transcription coactivator activity
- GO:0005739 mitochondrion
- GO:0004693 cyclin-dependent protein kinase activity
- GO:0005524 ATP binding
- GO:0005675 holo TFIIH complex
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006361 transcription initiation from RNA polymerase I promoter
- GO:0006362 transcription elongation from RNA polymerase I promoter
- GO:0006363 termination of RNA polymerase I transcription
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006370 mRNA capping
- GO:0006468 protein phosphorylation
- GO:0008022 protein C-terminus binding
- GO:0008094 DNA-dependent ATPase activity
- GO:0008283 cell proliferation
- GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
- GO:0016032 viral reproduction
- GO:0030521 androgen receptor signaling pathway
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0050434 positive regulation of viral transcription
- GO:0050681 androgen receptor binding
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of CDK7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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