CDK7 antibody | MO-1.1

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Anti CDK7 Antibody, clone MO-1.1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cdk7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to cdk7 (molecular weight 39 kDa)

Mouse anti CDK7

Product Type: PrecisionAb Monoclonal
Clone: MO-1.1
Isotype: IgG2b
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00023 Datasheet	C IP WB SDS	100 µl	Log in		Get Quote

Mouse anti Human cdk7 antibody, clone MO-1.1 recognizes human cyclin-dependent kinase 7, also known as 39 kDa protein kinase, cell division protein kinase 7, serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit.

Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).

Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.

Product Details

Target Species: Human
Western Blotting: Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant fragment of cdk7 corresponding to a 221 amino acid sequence from the C-terminus

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: 12 months from date of despatch

More Information

UniProt: P50613
Entrez Gene: CDK7
GO Terms: GO:0000079 regulation of cyclin-dependent protein kinase activity; GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000086 G2/M transition of mitotic cell cycle; GO:0000718 nucleotide-excision repair, DNA damage removal; GO:0003713 transcription coactivator activity; GO:0005739 mitochondrion; GO:0004693 cyclin-dependent protein kinase activity; GO:0005524 ATP binding; GO:0005675 holo TFIIH complex; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006361 transcription initiation from RNA polymerase I promoter; GO:0006362 transcription elongation from RNA polymerase I promoter; GO:0006363 termination of RNA polymerase I transcription; GO:0006367 transcription initiation from RNA polymerase II promoter; GO:0006368 transcription elongation from RNA polymerase II promoter; GO:0006370 mRNA capping; GO:0006468 protein phosphorylation; GO:0008022 protein C-terminus binding; GO:0008094 DNA-dependent ATPase activity; GO:0008283 cell proliferation; GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity; GO:0016032 viral reproduction; GO:0030521 androgen receptor signaling pathway; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0050434 positive regulation of viral transcription; GO:0050681 androgen receptor binding; GO:0051301 cell division
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of CDK7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

Application Based External Images

Western Blotting

Product Specific References

Source Reference

Tassan, J.P. et al. (1994) Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
J Cell Biol. 127 (2): 467-78.

References for CDK7 antibody

Aagaard, L. et al. (1995) Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines.
Int J Cancer. 61 (1): 115-20.
Falck, J. et al. (2001) Functional impact of concomitant versus alternative defects in the Chk2-p53 tumour suppressor pathway.
Oncogene. 20 (39): 5503-10.
Syljuåsen, R.G. et al. (2006) Adaptation to the ionizing radiation-induced G2 checkpoint occurs in human cells and depends on checkpoint kinase 1 and Polo-like kinase 1 kinases.
Cancer Res. 66 (21): 10253-7.

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