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CDK7 antibody | MO-1.1

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Anti CDK7 Antibody, clone MO-1.1 (PrecisionAb Monoclonal Antibody) thumbnail image 1
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Western blot analysis of whole cell lysates probed with cdk7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to cdk7 (molecular weight 39 kDa)

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Mouse anti CDK7

Product Type
PrecisionAb Monoclonal
Clone
MO-1.1
Isotype
IgG2b
Format
Purified
Specificity
CDK7

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Mouse anti Human cdk7 antibody, clone MO-1.1 recognizes human cyclin-dependent kinase 7, also known as 39 kDa protein kinase, cell division protein kinase 7, serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit.

Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).

Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant fragment of cdk7 corresponding to a 221 amino acid sequence from the C-terminus
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

Source Reference

  1. Tassan, J.P. et al. (1994) Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
    J Cell Biol. 127 (2): 467-78.

References for CDK7 antibody

  1. Aagaard, L. et al. (1995) Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines.
    Int J Cancer. 61 (1): 115-20.
  2. Falck, J. et al. (2001) Functional impact of concomitant versus alternative defects in the Chk2-p53 tumour suppressor pathway.
    Oncogene. 20 (39): 5503-10.
  3. Syljuåsen, R.G. et al. (2006) Adaptation to the ionizing radiation-induced G2 checkpoint occurs in human cells and depends on checkpoint kinase 1 and Polo-like kinase 1 kinases.
    Cancer Res. 66 (21): 10253-7.

Further Reading

  1. Lukás, J. et al. (1992) Distinct forms of human CDC2 identified by novel monoclonal antibodies.
    Eur J Biochem. 207 (1): 169-76.

Western Blotting

Synonyms
Cyclin-Dependent Kinase 7
MO15
UniProt
P50613
Entrez Gene
CDK7
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003713 transcription coactivator activity
GO:0005739 mitochondrion
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005675 holo TFIIH complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006362 transcription elongation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0006468 protein phosphorylation
GO:0008022 protein C-terminus binding
GO:0008094 DNA-dependent ATPase activity
GO:0008283 cell proliferation
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0016032 viral reproduction
GO:0030521 androgen receptor signaling pathway
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050434 positive regulation of viral transcription
GO:0050681 androgen receptor binding
GO:0051301 cell division

VMA00023

090714

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