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CDK7 antibody | MO-1.1

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Mouse anti Human CDK7

Product Type
Monoclonal Antibody
Clone
MO-1.1
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1993
Datasheet
C IP WB
MSDS
0.1 mg loader

Mouse anti Human Cdk7 antibody, clone MO-1.1 recognizes human Cyclin-dependent kinase 7, also known as 39 kDa protein kinase, Cell division protein kinase 7, Serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit. Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription.

Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A/G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
Immunogen
221 amino acid recombinant fragment of Cdk7 C terminus
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells derived from immunized Balb/c mice were fused with cells from the NS-2 mouse myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P50613
Entrez Gene
CDK7
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003713 transcription coactivator activity
GO:0005739 mitochondrion
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005675 holo TFIIH complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006362 transcription elongation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0006468 protein phosphorylation
GO:0008022 protein C-terminus binding
GO:0008094 DNA-dependent ATPase activity
GO:0008283 cell proliferation
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0016032 viral reproduction
GO:0030521 androgen receptor signaling pathway
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050434 positive regulation of viral transcription
GO:0050681 androgen receptor binding
GO:0051301 cell division
Regulatory
For research purposes only

Applications of CDK7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 1ug/ml 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
HeLa cells fixed with acetone/methanol
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Western Blotting

Product Specific References

References for CDK7 antibody

  1. Tassan, J.P. et al. (1994) Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
    J Cell Biol. 127 (2): 467-78.
  2. Syljuåsen, R.G. et al. (2006) Adaptation to the ionizing radiation-induced G2 checkpoint occurs in human cells and depends on checkpoint kinase 1 and Polo-like kinase 1 kinases.
    Cancer Res. 66: 10253-7.
  3. Aagaard, L. et al. (1995) Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines.
    Int J Cancer. 61 (1): 115-20.
  4. Falck, J. et al. (2001) Functional impact of concomitant versus alternative defects in the Chk2-p53 tumour suppressor pathway.
    Oncogene. 20: 5503-10.

Further Reading

  1. Lukás, J. et al. (1992) Distinct forms of human CDC2 identified by novel monoclonal antibodies.
    Eur J Biochem. 207 (1): 169-76.
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