CDK6 antibody

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Mouse anti CDK6

Cdk6 antibody recognizes cyclin-dependent kinase 6, also known as cell division protein kinase 6 or serine/threonine-protein kinase PLSTIRE. The antibody has been extensively validated for western blotting using whole cell lysates.

CDK6 Antibody with Control Lysate

Cdk6 antibody recognizes cyclin-dependent kinase 6, also known as cell division protein kinase 6 or serine/threonine-protein kinase PLSTIRE. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00022 WB 100 µl
VMA00022KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cdk6 antibody recognizes the human cyclin dependent kinase 6, also known as cdk6 or serine/threonine-protein kinase PLSTIRE. Cdk6 is a 326 amino acid member of the CDC2/CDKX subfamily containing a single protein kinase domain and plays an important role in cell cycle progression from the G1 to S phase (Sun et al. 2008).

Mouse anti Human cdk6 antibody recognizes cdk6 as a single band of ~37 kDa in multiple cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti Cdk6 antibody detects a band of approximately 37 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cdk6
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q00534 Related reagents
Entrez Gene
CDK6 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001726 ruffle
GO:0001954 positive regulation of cell-matrix adhesion
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0010468 regulation of gene expression
GO:0030332 cyclin binding
GO:0042063 gliogenesis
GO:0043697 cell dedifferentiation
GO:0045646 regulation of erythrocyte differentiation
GO:0045668 negative regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0048146 positive regulation of fibroblast proliferation
GO:0050680 negative regulation of epithelial cell proliferation
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CDK6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Product Specific References

References for CDK6 antibody

  1. Zhu, G. et al. (2016) Combination with γ-secretase inhibitor prolongs treatment efficacy of BRAF inhibitor in BRAF-mutated melanoma cells.
    Cancer Lett. 376 (1): 43-52.

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