CDK6 antibody

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Mouse anti CDK6

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00022
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human cdk6 antibody recognizes the human cyclin dependent kinase 6, also known as cdk6 or serine/threonine-protein kinase PLSTIRE. Cdk6 is a 326 amino acid member of the CDC2/CDKX subfamily containing a single protein kinase domain and plays an important role in cell cycle progression from the G1 to S phase (Sun et al. 2008).

Mouse anti Human cdk6 antibody recognizes cdk6 as a single band of ~37 kDa in multiple cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti Cdk6 antibody detects a band of approximately 37 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cdk6

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q00534 Related reagents
Entrez Gene
CDK6 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001726 ruffle
GO:0001954 positive regulation of cell-matrix adhesion
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0010468 regulation of gene expression
GO:0030332 cyclin binding
GO:0042063 gliogenesis
GO:0043697 cell dedifferentiation
GO:0045646 regulation of erythrocyte differentiation
GO:0045668 negative regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0048146 positive regulation of fibroblast proliferation
GO:0050680 negative regulation of epithelial cell proliferation
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CDK6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Product Specific References

References for CDK6 antibody

  1. Zhu, G. et al. (2016) Combination with γ-secretase inhibitor prolongs treatment efficacy of BRAF inhibitor in BRAF-mutated melanoma cells.
    Cancer Lett. 376 (1): 43-52.

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