CDK6 antibody | DCS-83.1

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Mouse anti Human CDK6

Product Type
Monoclonal Antibody
Clone
DCS-83.1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1992 C IP WB 0.1 mg
Mouse anti Human Cdk6 antibody, clone DCS-83 recognizes the human cyclin dependent kinase 6, also known as Cdk6 or Serine/threonine-protein kinase PLSTIRE. Cdk6 is a 326 amino acid member of the CDC2/CDKX subfamily containing a single protein kinase domain. Pck6 migrates at ~37kDa in Western blotting under reducing conditions. Cdk6 pays an important role in cell cycle progression from the G1 to S phase (Sun et al. 2008)

Mouse anti Human Cdk6, clone DCS83 additionally recognizes both the rat and mouse homologs of Human Cdk6. and has been successfully used for immunoprecipitation and immunofluorescesnce on acetone/methanol fixed cell preparations (Lukas et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A/G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
Immunogen
Recombinant cdk6 protein
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q00534 Related reagents
Entrez Gene
CDK6 Related reagents
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001726 ruffle
GO:0001954 positive regulation of cell-matrix adhesion
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006468 protein phosphorylation
GO:0010468 regulation of gene expression
GO:0030332 cyclin binding
GO:0042063 gliogenesis
GO:0043697 cell dedifferentiation
GO:0045646 regulation of erythrocyte differentiation
GO:0045668 negative regulation of osteoblast differentiation
GO:0045786 negative regulation of cell cycle
GO:0048146 positive regulation of fibroblast proliferation
GO:0050680 negative regulation of epithelial cell proliferation
GO:0051301 cell division
Regulatory
For research purposes only

Applications of CDK6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
U-2-OS sarcoma cell line

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for CDK6 antibody

  1. Lukas, C. et al. (1999) Immunohistochemical analysis of the D-type cyclin-dependent kinases Cdk4 and Cdk6, using a series of monoclonal antibodies.
    Hybridoma. 18: 225-34.
  2. Tsubari, M. et al. (1999) Hepatocyte growth factor releases mink epithelial cells from transforming growth factor beta1-induced growth arrest by restoring Cdk6 expression and cyclin E-associated Cdk2 activity.
    Mol Cell Biol. 19: 3654-63.

Further Reading

  1. Elledge, S.J. (1996) Cell cycle checkpoints: preventing an identity crisis.
    Science. 274 (5293): 1664-72.
Eukaryotic cell cycle diagram

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Understanding the Eukaryotic Cell Cycle - A Biological and Experimental Overview

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