CDC42 antibody | EF01/3E10

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Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CDC42 antibody (1/2,000, **VMA00651**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CDC42 (molecular weight 21 kDa).

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) gallery image 2 — Staining of formalin fixed, paraffin embedded human colon with Mouse anti Human CDC42 (**VMA00651**). Hematoxylin was used as nucleus counterstain.

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) gallery image 3 — Staining of formalin fixed, paraffin embedded human colon with Mouse anti Human CDC42 (**VMA00651**). Hematoxylin was used as nucleus counterstain.

Anti CDC42 Antibody, clone EF01/3E10 (PrecisionAb Monoclonal Antibody) gallery image 4 — Staining of formalin fixed, paraffin embedded human colon with Mouse anti Human CDC42 (**VMA00651**). Hematoxylin was used as nucleus counterstain.

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Mouse anti CDC42

Product Type: PrecisionAb Monoclonal
Clone: EF01/3E10
Isotype: IgG2b
Format: Purified
Specificity: CDC42

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00651 Datasheet Datasheet SDS SDS	P WB	100 µl		Log in	Get Quote
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Mouse anti Human CDC42 antibody recognizes cell division control protein 42 homolog, also known as G25K GTP-binding protein or growth-regulating protein, small GTP binding protein CDC42.

CDC42 is part of the Rho family of small GTPases which act as molecular switches by cycling between an active (GTP-bound) and an inactive (GDP-bound) state, regulated by a variety of proteins including guanine nucleotide exchange factors (GEFs). When activated, CDC42 interacts with effectors and controls multiple signaling pathways affecting cell cycle progression, morphology, and migration (Watson et al. 2017) including Raf-MEK-ERK and PI3K-Akt signaling, and plays a critical role in actin polymerization and extension, and cell polarity (Humphries-Bickley et al. 2017). Due to these functions, overexpression of CDC42 activation is oncogenic and contributes to metastasis (Qadir et al. 2015). Overexpression rather than mutation of CDC42 in a variety of cancers has led to the suggestion that it could be a useful target for inhibition in cancer therapy, or for overcoming resistance to cancer therapies (del Mar Maldonado and Dharmawardhane 2018)

Target Species: Human
Western Blotting: Mouse anti CDC42 antibody detects a band of approximately 21 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: E. coli-derived recombinant human CDC42 (aa 1-191)
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin		1/10	1/50
Western Blotting		1/1000	1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P60953
Entrez Gene: CDC42
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0003924 GTPase activity; GO:0030036 actin cytoskeleton organization; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0005525 GTP binding; GO:0007163 establishment or maintenance of cell polarity; GO:0007173 epidermal growth factor receptor signaling pathway; View More GO Terms; GO:0007264 small GTPase mediated signal transduction; GO:0007411 axon guidance; GO:0030175 filopodium; GO:0030225 macrophage differentiation; GO:0031274 positive regulation of pseudopodium assembly; GO:0031295 T cell costimulation; GO:0031333 negative regulation of protein complex assembly; GO:0031996 thioesterase binding; GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway; GO:0042692 muscle cell differentiation; GO:0051056 regulation of small GTPase mediated signal transduction; GO:0051149 positive regulation of muscle cell differentiation