CDC42 antibody | EF01/3E10

Mouse anti CDC42

Product Type
PrecisionAb Monoclonal
Clone
EF01/3E10
Isotype
IgG2b
Format
Purified
Specificity
CDC42

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VMA00651
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Mouse anti Human CDC42 antibody recognizes cell division control protein 42 homolog, also known as G25K GTP-binding protein or growth-regulating protein, small GTP binding protein CDC42.

CDC42 is part of the Rho family of small GTPases which act as molecular switches by cycling between an active (GTP-bound) and an inactive (GDP-bound) state, regulated by a variety of proteins including guanine nucleotide exchange factors (GEFs). When activated, CDC42 interacts with effectors and controls multiple signaling pathways affecting cell cycle progression, morphology, and migration (Watson et al. 2017) including Raf-MEK-ERK and PI3K-Akt signaling, and plays a critical role in actin polymerization and extension, and cell polarity (Humphries-Bickley et al. 2017). Due to these functions, overexpression of CDC42 activation is oncogenic and contributes to metastasis (Qadir et al. 2015). Overexpression rather than mutation of CDC42 in a variety of cancers has led to the suggestion that it could be a useful target for inhibition in cancer therapy, or for overcoming resistance to cancer therapies (del Mar Maldonado and Dharmawardhane 2018)
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti CDC42 antibody detects a band of approximately 21 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived recombinant human CDC42 (aa 1-191)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/10 1/50
Western Blotting 1/1000 1/2000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P60953
Entrez Gene
CDC42
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0030036 actin cytoskeleton organization
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0007163 establishment or maintenance of cell polarity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007264 small GTPase mediated signal transduction
GO:0007411 axon guidance
GO:0030175 filopodium
GO:0030225 macrophage differentiation
GO:0031274 positive regulation of pseudopodium assembly
GO:0031295 T cell costimulation
GO:0031333 negative regulation of protein complex assembly
GO:0031996 thioesterase binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0042692 muscle cell differentiation
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051149 positive regulation of muscle cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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