CDC34 antibody | AB02/4C3

Mouse anti CDC34

Product Type
PrecisionAb Monoclonal
Clone
AB02/4C3
Isotype
IgG2a
Format
Purified
Specificity
CDC34

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VMA00815
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IP WB 100 µl loader
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Mouse anti CDC34 antibody recognizes ubiquitin-conjugating enzyme E2 R1, also known as UBCH3, UBE2R1.

CDC34 is an E2 ubiquitin-conjugating enzyme involved with ubiquitin signaling, one of the main mechanisms of reversible post-translational modification of proteins (Williams et al. 2019). CDC34 plays a role in cell cycle progression by facilitating degradation of several cyclin-dependent kinase inhibitors (Zhang and Sun 2020). Furthermore, CDC34 also controls cell cycle progression at G1 by mediating degradation of p27KIP1 (Han et al. 2018). Overexpression of CDC34 has been identified in several human cancers. Its overexpression has been shown to promote growth and survival of lung cancer cells, whereas its knockdown inhibited these processes. In patients, CDC34 overexpression appears to correlate with worse prognosis (Zhang and Sun 2020).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti CDC34 antibody detects a band of approximately 31 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-236 of human CDC34
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P49427
Entrez Gene
CDC34
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000209 protein polyubiquitination
GO:0005515 protein binding
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006270 DNA-dependent DNA replication initiation
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0043951 negative regulation of cAMP-mediated signaling
GO:0051246 regulation of protein metabolic process
GO:0070936 protein K48-linked ubiquitination
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