CDC34 antibody | AB02/4C3
siRNA Validated
Mouse anti CDC34
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB02/4C3
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- CDC34
Antibody Quality and Performance Guarantee
| Mouse anti CDC34 antibody recognizes ubiquitin-conjugating enzyme E2 R1, also known as UBCH3, UBE2R1. CDC34 is an E2 ubiquitin-conjugating enzyme involved with ubiquitin signaling, one of the main mechanisms of reversible post-translational modification of proteins (Williams et al. 2019). CDC34 plays a role in cell cycle progression by facilitating degradation of several cyclin-dependent kinase inhibitors (Zhang and Sun 2020). Furthermore, CDC34 also controls cell cycle progression at G1 by mediating degradation of p27KIP1 (Han et al. 2018). Overexpression of CDC34 has been identified in several human cancers. Its overexpression has been shown to promote growth and survival of lung cancer cells, whereas its knockdown inhibited these processes. In patients, CDC34 overexpression appears to correlate with worse prognosis (Zhang and Sun 2020). |
|
- Target Species
- Human
- Western Blotting
- Mouse anti CDC34 antibody detects a band of approximately 31 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-236 of human CDC34
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
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| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P49427
- Entrez Gene
- CDC34
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000209 protein polyubiquitination
- GO:0005515 protein binding
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006270 DNA-dependent DNA replication initiation
- GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
- View More GO Terms
- GO:0043687 post-translational protein modification
- GO:0043951 negative regulation of cAMP-mediated signaling
- GO:0051246 regulation of protein metabolic process
- GO:0070936 protein K48-linked ubiquitination
VMA00815
100004553
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