Cdc25C antibody

100% Secure


Mouse anti Cdc25C

CDC25C antibody recognizes M-phase inducer phosphatase 3, also known as dual specificity phosphatase CDC25C, mitosis inducer CDC25, phosphotyrosine phosphatase, protein phosphatase 1 or regulatory subunit 60. This antibody has been extensively validated for western blotting using whole cell lysates.

Cdc25C Antibody with Control Lysate

CDC25C antibody recognizes M-phase inducer phosphatase 3, also known as dual specificity phosphatase CDC25C, mitosis inducer CDC25, phosphotyrosine phosphatase, protein phosphatase 1 or regulatory subunit 60. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00221 WB 100 µl
VMA00221KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human Cdc25C antibody recognizes the M-phase inducer phosphatase 3, also known as M-phase inducer phosphatase 3, dual specificity phosphatase Cdc25C, mitosis inducer CDC25, phosphotyrosine phosphatase, protein phosphatase 1 and regulatory subunit 60.

The CDC25C gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described, however, the full-length nature of many of them is not known (provided by RefSeq, Jul 2008).

Mouse anti Human Cdc25C antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human Cdc25C expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P30307 Related reagents
Entrez Gene
CDC25C Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000075 cell cycle checkpoint
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005829 cytosol
GO:0004725 protein tyrosine phosphatase activity
GO:0005654 nucleoplasm
GO:0006470 protein dephosphorylation
GO:0007067 mitosis
GO:0007088 regulation of mitosis
GO:0007089 traversing start control point of mitotic cell cycle
GO:0008283 cell proliferation
GO:0044419 interspecies interaction between organisms
GO:0050699 WW domain binding
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cdc25C antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti Cdc25C detects a band of approximately 75 kDa in HEK293 cell lysates
Western Blotting
Anti Cdc25C detects a band of approximately 75 kDa in HEK293 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB