CDC20 antibody | GH02/4F3
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation (provided by RefSeq, Jul 2008).
Mouse anti Human CDC20 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Mouse anti CDC20 antibody detects a band of approximately 52 kDa in HeLa cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- E. coli-derived recombinant human CDC20 (aa 1-300)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- As supplied, 12 months from date of despatch
- Q12834 Related reagents
- Entrez Gene
- CDC20 Related reagents
- GO Terms
- GO:0000236 mitotic prometaphase
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0005819 spindle
- GO:0007094 mitotic cell cycle spindle assembly checkpoint
- GO:0008022 protein C-terminus binding
- GO:0019899 enzyme binding
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0051301 cell division
- GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051488 activation of anaphase-promoting complex activity
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- For research purposes only
Applications of CDC20 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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