CD93 antibody | X-2

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Mouse anti Human CD93

Product Type
Monoclonal Antibody
Clone
X-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1207 C F IP 0.1 mg
Mouse anti Human CD93 antibody, clone X-2 recognizes human Complement component C1q receptor, also known as CD93, C1q/MBL/SPA receptor, C1qR or Complement component 1 q subcomponent receptor 1. CD93 is a 652 amino acid ~126 kDa single pass type 1 transmembrane glycoprotein containing a single C-type lectin domain and multiple EGF-like domains. CD93 is expressed on granulocytes, monocytes and endothelial cells, but is not expressed on tissue macrophages (Nepomuceno andTenner 1998).

Culture of monocytes in the presence of IL-4 and GM-CSF to produce immature dendritic cells express surface CD93 but maturation in the presence of LPS or ionomycin leads to down regulation of the CD93 antigen expression (Steinberger et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1%Bovine Serum Albumin
0.01%Thiomersal
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q9NPY3 Related reagents
Entrez Gene
CD93 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0001849 complement component C1q binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
GO:0006909 phagocytosis
GO:0016337 cell-cell adhesion
GO:0042116 macrophage activation
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of CD93 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Immunohistology - Frozen 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD93 antibody

  1. McGreal, E.P. et al. (2002) Human C1qRp is identical with CD93 and the mNI-11 antigen but does not bind C1q.
    J Immunol. 168 (10): 5222-32.
  2. Steinberger, P. et al. (2002) Identification of human CD93 as the phagocytic C1q receptor (C1qRp) by expression cloning.
    J Leukoc Biol. 71: 133-40.