CD92 antibody | VIM-15b
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Mouse anti Human CD92
- Product Type
- Monoclonal Antibody
- Clone
- VIM-15b
- Isotype
- IgG2b
- Specificity
- CD92
Antibody Quality and 
Unrivaled 
| Mouse anti human CD92 antibody, clone VIM-15b recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70 kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine. Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S. et al.). Removal of Sodium Azide is recommended prior to use in functional assays. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA2521T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA2521: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- MV4-11 acute monocyte leukaemia cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD92 antibody
-
Wille, S. et al. (2001) Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells.
J Immunol. 167 (10): 5795-804. -
Majdic, O. et al. (1995) Leucocyte Typing V.
Oxford University Press, Oxford pp.984-5.
Further Reading
-
O'regan, S. et al. (2000) An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins.
Proc Natl Acad Sci U S A. 97 (4): 1835-40.
- Synonyms
- CTL1
- RRID
- AB_961480
- UniProt
- Q8WWI5
- Entrez Gene
- SLC44A1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005741 mitochondrial outer membrane
- GO:0015220 choline transmembrane transporter activity
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