CD92 antibody | VIM-15b

Mouse anti Human CD92

Product Type
Monoclonal Antibody
Clone
VIM-15b
Isotype
IgG2b
Specificity
CD92

Product Code Applications Pack Size List Price Your Price Qty
MCA2521T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 25 µg loader
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loader
MCA2521
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Mouse anti human CD92 antibody, clone VIM-15b recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70 kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.

CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.

Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S. et al.). Removal of Sodium Azide is recommended prior to use in functional assays.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA2521T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA2521: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
MV4-11 acute monocyte leukaemia cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil.

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References for CD92 antibody

  1. Wille, S. et al. (2001) Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells.
    J Immunol. 167 (10): 5795-804.
  2. Majdic, O. et al. (1995) Leucocyte Typing V.
    Oxford University Press, Oxford pp.984-5.

Further Reading

  1. O'regan, S. et al. (2000) An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins.
    Proc Natl Acad Sci U S A. 97 (4): 1835-40.

Synonyms
CTL1
RRID
AB_961480
UniProt
Q8WWI5
Entrez Gene
SLC44A1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005741 mitochondrial outer membrane
GO:0015220 choline transmembrane transporter activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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