CD91 antibody | A2Mr alpha-2
Mouse anti Human CD91 antibody, clone A2Mr alpha-2 (MCA1965) used to evaluate CD91 levels in an &alppha;2M column flow through by western blotting.
Image caption:
Purified sLRP1 inhibits DENV2 infection.
(A) Chromatographic profile of the affinity chromatography using receptor-activated α2M as immobilized ligand. PT: pass-through. Numbers in parenthesis identify the lane in SDS-PAGE (B) and western blotting analysis. In (C) membrane was probed with an anti-LRP1 Mab (MCA1965) that recognizes the alpha chain of the receptor. (C). Microsomal fraction (FM) of Huh7 cells was used as control of migration of α chain of LRP1. (D) Binding analysis of purified sLRP1 to recombinant RAP protein using SPR. RAP protein was immobilized on the surface of the chip and purified sLRP1 was injected diluted in HBS-Ca at 1 μg/ml. (E) Inhibition of DENV2 infection in Huh7 cells after incubation with chromatography fractions. Results from one representative experiment out of three independent experiments. Error bars represent the range of three sample replicates.
From: Huerta V, Ramos Y.
Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry.
Methods Mol Biol. 2022;2409:133-153.
doi: 10.1007/978-1-0716-1879-0_10.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD91:RPE
- Product Type
- Monoclonal Antibody
- Clone
- A2Mr alpha-2
- Isotype
- IgG1
- Specificity
- CD91
Antibody Quality and Performance Guarantee
| Mouse anti Human CD91 antibody, clone A2Mr alpha-2 recognizes human CD91, also known as Prolow-density lipoprotein receptor-related protein 1, Alpha-2-macroglobulin receptor or apolipoprotein E receptor. CD91 is a 4525 amino acid protein post translationally cleaved into 3 subunits, a 85 kDa type I transmembrane carboxyl chain (LRP85) non-covalently bound to a 515 kDa extracellular N-terminal subunit (LRP515)containing multiple EGF-like and LDL-receptor Class A and Class B domains. Additionally, there is an intracellular domain (LRPICD) which can be cleaved from the transmambrane domain by gamma secretase (May et al. 2004). Clone A2Mr alpha-2 detects an epitope within the LRP515 chain. CD91 is a multifunctional protein involved in processes inluding the phagocytosis and endocytosis of apoptotic cells (Nilsson et al. 2012), clearance of activated serum alpha-2-macroglobulin (Kristensen et al. 1990), modulation of the inflammatory response (Staudt et al. 2013) and acts as a receptor for Pseudomonas aeruginosa exotoxin A (Kounnas et al. 1992). Mouse anti Human CD91, clone A2Mr alpha-2 has been used extensively for the detection of CD91 by flow cytometry and immunohistochemistry on formalin fixed paraffin embedded tissues (Bourazopoulou et al. 2009). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Purified alpha2 macroglobulin receptor
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG1 Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD91 antibody
-
Moestrup, S.K. et al. (1992) Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.
Cell Tissue Res. 269 (3): 375-82. -
Moestrup, S.K. & Hokland, P. (1992) Surface expression of the alpha 2-macroglobulin receptor on human malignant blood cells.
Leuk Res. 16 (3): 227-34. -
Hvidberg, V. et al. (2005) Identification of the receptor scavenging hemopexin-heme complexes.
Blood. 106: 2572-9. -
Hodge, S. et al. (2006) Azithromycin increases phagocytosis of apoptotic bronchial epithelial cells by alveolar macrophages.
Eur Respir J. 28 (3): 486-95. -
Hodge, S. et al. (2007) Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease.
Am J Respir Cell Mol Biol. 37: 748-55. -
Pawluczyk, I.Z. et al. (2008) Perindoprilat modulates the activity of lipoprotein receptor-related protein in human mesangial cells.
J Biol Chem. 283: 4588-94. -
Bourazopoulou, E. et al. (2009) Functional expression of the alpha 2-macroglobulin receptor CD91 in salivary gland epithelial cells.
J Autoimmun. 33: 141-6. -
Chen, J.S. et al. (2010) Secreted Heat Shock Protein 90{alpha} Induces Colorectal Cancer Cell Invasion through CD91/LRP-1 and NF-{kappa}B-mediated Integrin {alpha}V Expression.
J Biol Chem. 285: 25458-66.
View The Latest Product References
-
Cunnington, A.J. et al. (2012) Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
J Immunol. 189 (11): 5336-46. -
Huerta, V. et al. (2020) The low-density lipoprotein receptor-related protein-1 is essential for Dengue virus infection
bio&χiv 10 Jun [Epub ahead of print].
- Synonyms
- ALPHA-2 Macroglobulin Receptor
- RRID
- AB_323268
- UniProt
- Q07954
- Entrez Gene
- LRP1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005634 nucleus
- GO:0005905 coated pit
- GO:0007275 multicellular organismal development
- GO:0010875 positive regulation of cholesterol efflux
- GO:0030178 negative regulation of Wnt receptor signaling pathway
- GO:0032403 protein complex binding
- View More GO Terms
- GO:0032429 regulation of phospholipase A2 activity
- GO:0034187 apolipoprotein E binding
- GO:0042954 lipoprotein transporter activity
- GO:0043277 apoptotic cell clearance
- GO:0070325 lipoprotein particle receptor binding
MCA1965PE
150607
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