CD9 antibody | 72F6

100% Secure

Mouse anti Human CD9

Product Type
Monoclonal Antibody
Clone
72F6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1805T C P* 0.1 ml
Mouse anti Human CD9 antibody, clone 72F6 recognizes the human CD9 cell surface antigen, also known as 5H9 antigen, MIC3, MRP-1, Tetraspanin-29 or p24. CD9 is a ~24-27 kDa multi-pass transmembrane glycoprotein, a member of the tetraspanin family, expressed by B cells, monocytes, platelets and by neurons and glial cells.

In melanoma and breast cancer CD9 antigen expression may be linked to a favourable prognosis (Si et al. 1993).

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
15mMSodium Azide
Immunogen
Recombinant human CD9.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P21926 Related reagents
Entrez Gene
CD9 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0030168 platelet activation
GO:0030913 paranodal junction assembly
GO:0031092 platelet alpha granule membrane
Regulatory
For research purposes only

Applications of CD9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/75 1/150
Immunohistology - Paraffin 1 1/20 1/40
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Tris/EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

Product Specific References

References for CD9 antibody

  1. Gullberg, E. et al. (2006) Identification of cell adhesion molecules in the human follicle-associated epithelium that improve nanoparticle uptake into the Peyer's patches.
    J Pharmacol Exp Ther. 319: 632-9.
  2. Kischel, P. et al. (2012) Overexpression of CD9 in Human Breast Cancer Cells Promotes the Development of Bone Metastases.
    Anticancer Res. 32: 5211-20.
  3. Miyake, M. et al. (2016) The Pro-inflammatory Cytokine Interleukin-6 Regulates Nanoparticle Transport Across Model Follicle-Associated Epithelium Cells.
    J Pharm Sci. 105 (7): 2099-104.