CD81 antibody | AbD06084

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Human anti Human CD81

Product Type
Monoclonal Antibody
Clone
AbD06084
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA087A E F 50 µg
HCA087 E F 0.1 mg
Human anti Human CD81, clone AbD06084 recognizes human CD81, a 26 kDa member of the TM4SF tetraspanin family. In humans, CD81 is widely expressed on most tissues with T-cells having the highest level of expression, followed by monocytes and B-cells. Levels of CD81 expression are increased after activation.

CD81 has the ability to interact with a wide range of proteins and plays an important role in a variety of leucocyte functions including activation, proliferation and differentiation. In recent studies, the CD81 molecule has been identified as the putative receptor for the hepatitis C virus (HCV) envelope E2 glycoprotein and is vital for HCV entry into hepatic cells.

Product Details

Target Species
Human
Product Form
Pack Size: 50 µg
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Pack Size: 0.1 mg
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 50 µg
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Pack Size: 0.1 mg
0.01% Thiomersal
Immunogen
Recombinant human CD81
Approx. Protein Concentrations
Pack Size: 50 µg
Ig concentration 0.1 mg/ml after reconstitution
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Pack Size: 50 µg
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.1 mg
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P60033 Related reagents
Entrez Gene
CD81 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0008104 protein localization
GO:0005887 integral to plasma membrane
GO:0006661 phosphatidylinositol biosynthetic process
GO:0008283 cell proliferation
GO:0008284 positive regulation of cell proliferation
GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050776 regulation of immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CD81 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry Neat 1/10 Pack Size: 50 µg
1/25 Pack Size: 0.1 mg
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Alexa Fluor® 488 MCA1396A488 100 Tests/1ml F
Mouse anti Histidine Tag:Alexa Fluor® 647 MCA1396A647 1 ml F
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti Histidine Tag:FITC MCA1396F 0.1 mg F IF
Mouse anti Histidine Tag:HRP MCA1396P 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Alexa Fluor® 488 MCA2200A488 100 Tests/1ml F*
Mouse anti c-Myc:Alexa Fluor® 647 MCA2200A647 100 Tests/1ml F*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:FITC MCA2200F 0.1 mg F*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti c-Myc:RPE MCA2200PE 100 Tests WB