CD80 antibody | OTI2B11

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Mouse anti CD80

CD80 antibody recognizes the T-lymphocyte activation antigen CD80, also known as B-lymphocyte activation antigen B7, CTLA-4 counter-receptor B7.1, activation B7-1 antigen, or costimulatory molecule variant IgV-CD80.

CD80 Antibody with Control Lysate

CD80 antibody recognizes the T-lymphocyte activation antigen CD80, also known as B-lymphocyte activation antigen B7, CTLA-4 counter-receptor B7.1, activation B7-1 antigen, or costimulatory molecule variant IgV-CD80.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2B11
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00138 WB 100 µl
VMA00138KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human CD80 antibody recognizes CD80, also known as B-lymphocyte activation antigen B7, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, activation B7-1 antigen, costimulatory factor CD80 and costimulatory molecule variant IgV-CD80.

CD80 is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy (provided by RefSeq, Aug 2011).

Mouse anti Human CD80 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD80 detects a band of approximately 60 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human CD80 (NP_005182) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P33681 Related reagents
Entrez Gene
CD80 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0035556 intracellular signal transduction
GO:0007267 cell-cell signaling
GO:0009967 positive regulation of signal transduction
GO:0015026 coreceptor activity
GO:0016563 transcription activator activity
GO:0031295 T cell costimulation
GO:0042110 T cell activation
GO:0044419 interspecies interaction between organisms
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045425 positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
GO:0045627 positive regulation of T-helper 1 cell differentiation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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