CD80 antibody | OTI2B11

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Anti CD80 Antibody, clone OTI2B11 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CD80 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 60 second exposure. Arrow points to CD80 (molecular weight 60 kDa)

Mouse anti CD80

CD80 antibody recognizes the T-lymphocyte activation antigen CD80, also known as B-lymphocyte activation antigen B7, CTLA-4 counter-receptor B7.1, activation B7-1 antigen, or costimulatory molecule variant IgV-CD80.

CD80 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI2B11
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00138	WB	100 µl
VMA00138KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human CD80 antibody recognizes CD80, also known as B-lymphocyte activation antigen B7, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, activation B7-1 antigen, costimulatory factor CD80 and costimulatory molecule variant IgV-CD80.

CD80 is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy (provided by RefSeq, Aug 2011).

Mouse anti Human CD80 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti CD80 detects a band of approximately 60 kDa in Raji cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human CD80 (NP_005182) produced in HEK293T cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P33681 Related reagents
Entrez Gene: CD80 Related reagents
GO Terms: GO:0005515 protein binding; GO:0016021 integral to membrane; GO:0035556 intracellular signal transduction; GO:0007267 cell-cell signaling; GO:0009967 positive regulation of signal transduction; GO:0015026 coreceptor activity; GO:0016563 transcription activator activity; GO:0031295 T cell costimulation; GO:0042110 T cell activation; GO:0044419 interspecies interaction between organisms; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045425 positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; GO:0045627 positive regulation of T-helper 1 cell differentiation; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of CD80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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