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CD8/CD4 | LT8 / RPA-T4

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Mouse anti Human CD8:FITC/CD4:RPE

Product Type
Dual Color Reagent
Clone
LT8 / RPA-T4
Isotype
Cocktail
Specificity
CD8/CD4

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DC011
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Mouse anti Human CD8:FITC/CD4:RPE Dual Color reeagent may be used to simultaneously identify CD4 and CD8 positive cells.

Clone LT8 recognises the human CD8 antigen, a ~32 kD dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells.

RPA-T4 is specific for the human CD4 antigen, a ~59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T cells, and weakly by monocytes.

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 100ul lysed whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC012 F 50 Tests loader
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Description Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD8/CD4

  1. Steele, J.C. et al. (2002) Detection of CD4(+)- and CD8(+)-T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma interferon release.
    J Virol. 76 (12): 6027-36.

RRID
AB_1125293
UniProt
P10966
P01730
P01732
Entrez Gene
CD4
CD8A
CD8B
GO Terms
GO:0001948 glycoprotein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005201 extracellular matrix structural constituent
GO:0005769 early endosome
GO:0005789 endoplasmic reticulum membrane
GO:0006948 induction by virus of host cell-cell fusion
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0015026 coreceptor activity
GO:0019059 initiation of viral infection
GO:0019901 protein kinase binding
GO:0030260 entry into host cell
GO:0031295 T cell costimulation
GO:0032507 maintenance of protein location in cell
GO:0042101 T cell receptor complex
GO:0042289 MHC class II protein binding
GO:0042803 protein homodimerization activity
GO:0045058 T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045234 protein palmitoleylation
GO:0045860 positive regulation of protein kinase activity
GO:0050690 regulation of defense response to virus by virus
GO:0050852 T cell receptor signaling pathway
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0019882 antigen processing and presentation
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response

DC011

156924

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