Mouse anti Human CD8:FITC/CD4:RPE Dual Color reeagent may be used to simultaneously identify CD4 and CD8 positive cells.
Clone LT8 recognises the human CD8 antigen, a ~32 kD dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells.
RPA-T4 is specific for the human CD4 antigen, a ~59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T cells, and weakly by monocytes.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
transmembrane receptor activity
transmembrane receptor protein tyrosine kinase signaling pathway
extracellular matrix structural constituent
endoplasmic reticulum membrane
induction by virus of host cell-cell fusion
zinc ion binding
initiation of viral infection
protein kinase binding
entry into host cell
T cell costimulation
maintenance of protein location in cell
T cell receptor complex
MHC class II protein binding
protein homodimerization activity
T cell selection
positive regulation of interleukin-2 biosynthetic process
positive regulation of protein kinase activity
regulation of defense response to virus by virus
T cell receptor signaling pathway
integral to plasma membrane
antigen processing and presentation
MHC class I protein binding
regulation of immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul to label 100ul lysed whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for CD8/CD4
Steele, J.C. et al. (2002) Detection of CD4(+)- and CD8(+)-T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma interferon release.
J Virol. 76 (12): 6027-36.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra