CD8/CD4 | LT8/RPA-T4

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Mouse anti Human CD8:FITC/CD4:RPE

Product Type: Dual Color Reagent
Clone: LT8/RPA-T4
Isotype: Cocktail

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
DC011	F			50 Tests

Mouse anti Human CD8:FITC/CD4:RPE Dual Color reeagent may be used to simultaneously identify CD4 and CD8 positive cells.

Clone LT8 recognises the human CD8 antigen, a ~32 kD dimer expressed by a subset of T cells with cytotoxic/suppressor activity, and more weakly by NK cells.

RPA-T4 is specific for the human CD4 antigen, a ~59 kDa cell surface glycoprotein expressed by the helper-inducer subset of T cells, and weakly by monocytes.

Product Details

Target Species

Human

Product Form

Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

Reconstitution

Reconstitute with 0.5 ml distilled water

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Antibody Isotypes

FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P10966 Related reagents; P01730 Related reagents; P01732 Related reagents
Entrez Gene: CD4 Related reagents; CD8A Related reagents; CD8B Related reagents
GO Terms: GO:0001948 glycoprotein binding; GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0004888 transmembrane receptor activity; GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway; GO:0005201 extracellular matrix structural constituent; GO:0005769 early endosome; GO:0005789 endoplasmic reticulum membrane; GO:0006948 induction by virus of host cell-cell fusion; GO:0006955 immune response; GO:0008270 zinc ion binding; GO:0015026 coreceptor activity; GO:0019059 initiation of viral infection; GO:0019901 protein kinase binding; GO:0030260 entry into host cell; GO:0031295 T cell costimulation; GO:0032507 maintenance of protein location in cell; GO:0042101 T cell receptor complex; GO:0042289 MHC class II protein binding; GO:0042803 protein homodimerization activity; GO:0045058 T cell selection; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045234 protein palmitoleylation; GO:0045860 positive regulation of protein kinase activity; GO:0050690 regulation of defense response to virus by virus; GO:0050852 T cell receptor signaling pathway; GO:0005576 extracellular region; GO:0005887 integral to plasma membrane; GO:0019882 antigen processing and presentation; GO:0042288 MHC class I protein binding; GO:0050776 regulation of immune response
Regulatory: For research purposes only

Applications of CD8/CD4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul to label 100ul lysed whole blood.

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control	DC012	50 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for CD8/CD4

Steele, J.C. et al. (2002) Detection of CD4(+)- and CD8(+)-T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma interferon release.
J Virol. 76 (12): 6027-36.

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