CD8/CD38/CD3/HLA DR | LT8 / AT13/5 / UCHT1 / YE2/36-HLK

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Four-Color Human CD8/CD38/CD3/HLA DR Flow Kit

Product Type
Four Color Reagent
Clone
LT8 / AT13/5 / UCHT1 / YE2/36-HLK
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4C004 F 50 Tests/0.5ml
Four-Color Human Flow Kit, CD8/CD38/CD3/HLA DR, clones LT8 / AT13/5 / UCHT1 / YE2/36-HLK can be used for single-step identification of human activated suppressor/cytotoxic (CD3+CD8+CD38+ and CD3+CD8+HLA DR+) T-cell subsets, useful in the study of Acquired Immunodeficiency Syndrome (AIDS) and other viral infection including Epstein Barr Virus (EBV) and Cytomegalovirus (CMV).

CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.

CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human suppressor/cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. An increase in CD8+CD38+ T-cells is a useful indicator of disease progression in HIV infection. This same subset of activated T-cells is also increased in other active viral infections such as EBV and CMV.

HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36kDa alpha and 27kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.

Product Details

Target Species
Human
Product Form
Four color combination consisting of APC, FITC, RPE-Cy5.5 and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.

APC conjugated CD8 (Mouse IgG1)
FITC conjugated CD38 (Mouse IgG1)
RPE-Cy5.5 conjugated CD3 (Mouse IgG1)
RPE conjugated HLA DR (Rat IgG2a)
Reconstitution
Reconstitute with 0.5ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P07766 Related reagents
P01732 Related reagents
P28907 Related reagents
Entrez Gene
CD8A Related reagents
CD3E Related reagents
CD38 Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0005886 plasma membrane
GO:0003953 NAD+ nucleosidase activity
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005488 binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0042493 response to drug
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030890 positive regulation of B cell proliferation
GO:0043066 negative regulation of apoptosis
GO:0050853 B cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CD8/CD38/CD3/HLA DR

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04B 100 Test/20ml F
Erythrolyse Red Blood Cell Lysing Buffer (10x) BUF04C 500 Tests/100ml F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Flow Cytometry Absolute Count Standard™ FCSC580 20 Tests F