CD8/CD38/CD3/HLA DR | LT8 / AT13/5 / UCHT1 / YE2/36-HLK
Four-Color Human Flow Kit, CD8/CD38/CD3/HLA DR, clones LT8 / AT13/5 / UCHT1 / YE2/36-HLK can be used for single-step identification of human activated cytotoxic (CD3+CD8+CD38+ and CD3+CD8+HLA DR+) T-cell subsets, useful in the study of Acquired Immunodeficiency Syndrome (AIDS) and other viral infection including Epstein Barr Virus (EBV) and Cytomegalovirus (CMV).
CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.
CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.
CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. An increase in CD8+CD38+ T-cells is a useful indicator of disease progression in HIV infection. This same subset of activated T-cells is also increased in other active viral infections such as EBV and CMV.
HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36kDa alpha and 27kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.
CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.
CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.
CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. An increase in CD8+CD38+ T-cells is a useful indicator of disease progression in HIV infection. This same subset of activated T-cells is also increased in other active viral infections such as EBV and CMV.
HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36kDa alpha and 27kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.
Product Details
- Target Species
- Human
- Product Form
- Four color combination consisting of APC, FITC, RPE-Cy5.5 and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
APC conjugated CD8 (Mouse IgG1)
FITC conjugated CD38 (Mouse IgG1)
RPE-Cy5.5 conjugated CD3 (Mouse IgG1)
RPE conjugated HLA DR (Rat IgG2a) - Reconstitution
- Reconstitute with 0.5ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P07766
- P01732
- P28907
- Entrez Gene
- CD8A
- CD3E
- CD38
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
- GO:0005886 plasma membrane
- GO:0003953 NAD+ nucleosidase activity
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005488 binding
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0042493 response to drug
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0043066 negative regulation of apoptosis
- GO:0050853 B cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of CD8/CD38/CD3/HLA DR
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Erythrolyse Red Blood Cell Lysing Buffer (10x) | BUF04B | F | 100 Test/20ml |
|
|
| Erythrolyse Red Blood Cell Lysing Buffer (10x) | BUF04C | F | 500 Tests/100ml |
|
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| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Flow Cytometry Absolute Count Standard™ | FCSC580 | F | 20 Tests |
