CD79a/CD5 | ZL7-4/MF7-14.5

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Mouse anti Human CD79a:FITC/CD5:RPE

Product Type
Dual Color Reagent
Clone
ZL7-4/MF7-14.5
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC030 F 50 Tests
Mouse anti Human CD79a:FITC/CD5:RPE dual color reagent is intended for the simultaneous evaluation of CD79a and CD5 on human cells.

CD79a is part of the B cell receptor complex, and is expressed by all B lymphocytes.

CD5 is a ~67 kDa glycoprotein expressed by all mature T cells and by a subset of mature B cells. CD5 has been shown to act as a ligand for the B cell marker CD72. CD5 positive B cells have been implicated in the pathogenesis of various autoimmune disorders and are the normal counterpart of the malignant cells in B cell chronic lymphocytic leukaemia.

Mouse anti Human CD79a:FITC/CD5:RPE may find particular application in the study of CD5 positive B lymphocytes and in the study of chronic lymphoproliferative diseases. The CD79a specific antibody in this combination has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma, see (Bell et al. 1999).

Product Details

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (MOUSE)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P06127 Related reagents
P11912 Related reagents
Entrez Gene
CD5 Related reagents
CD79A Related reagents
GO Terms
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
GO:0008037 cell recognition
GO:0008283 cell proliferation
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005771 multivesicular body
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0030183 B cell differentiation
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050853 B cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CD79a/CD5

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 100ul of whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1:FITC/Mouse IgG2a:RPE Negative Control DC029 50 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD79a/CD5

  1. Bell, P.B. et al. (1999) CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase.
    Cytometry. 38 (3): 102-5.