CD77 antibody | 38-13

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Rat anti Human CD77

Product Type
Monoclonal Antibody
Clone
38-13
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA579 F 100 Tests
Rat anti Human CD77 antibody, clone 38-13 recognizes the Gb3 - specific sugar sequence Gal alpha 1-4 Gal beta- 4G1c, also known as the blood group substance pk (CD77).

CD77 expression amongst leucocytes is restricted to the B cell lineage, and only cells at a certain maturation stage express the antigen.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by gel filtration from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Daudi cell line.
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the SP2/0-Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of CD77 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5 x 105 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Product Specific References

References for CD77 antibody

  1. Wiels, J. et al. (1981) Monoclonal antibody against a Burkitt lymphoma-associated antigen.
    Proc Natl Acad Sci U S A. 78 (10): 6485-8.
  2. Wiels, J. et al. (1984) Properties of immunotoxins against a glycolipid antigen associated with Burkitt's lymphoma.
    Cancer Res. 44: 129-133.
  3. Nudelman, E. et al. (1983) A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody.
    Science 220: 509-511.
  4. Fellous, M. et al. (1985) A monoclonal antibody against a Burkitt lymphoma associated antigen has an anti-Pk red blood cell specificity.
    Brit. J. Haematol. 60: 559-565.
  5. Wiels, J. and Tursz, T. (1989) BLA a glycolipid marker of Burkitt's lymphoma and a subset of germinal-centre B-cells.
    In Leucocyte Typing IV. Knapp, W. Ed. Oxford University Press.
  6. Yu, D. et al. (2007) Axon growth and guidance genes identify T-dependent germinal centre B cells.
    Immunol Cell Biol. 86: 3-14.
  7. Stamm, I. et al. (2008) Epithelial and mesenchymal cells in the bovine colonic mucosa differ in their responsiveness to Escherichia coli Shiga toxin 1.
    Infect Immun. 76: 5381-91.
  8. Shaknovich, R. et al. (2011) DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation.
    Blood. 118: 3559-69.
  9. Girard, M.C. et al. (2015) Prevention of renal damage caused by Shiga toxin type 2: Action of Miglustat on human endothelial and epithelial cells.
    Toxicon. 105: 27-33.
  10. Bangari, D.S. et al. (2015) &alphA;-Galactosidase A knockout mice: progressive organ pathology resembles the type 2 later-onset phenotype of Fabry disease.
    Am J Pathol. 185 (3): 651-65.