CD73 antibody | 2B6

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Mouse anti Human CD73

Product Type
Monoclonal Antibody
Clone
2B6
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4622GA E WB 0.1 mg
Mouse anti Human CD73, clone 2B6 recognizes human CD73, otherwise known as ecto-5'-nucleotidase, a GPI-anchored enzyme and member of the 5'-nucleotidase family, which catalyzes the dephosphorylation of 5'-mononucleotides into their corresponding nucleosides, and can also mediate co-stimulatory signals in T cell activation (Dianzani et al. 1993).

CD73 is expressed by subpopulations of T and B lymphocytes, and increases during lymphocyte development, acting as a lymphocyte maturation marker. A decrease in CD73 activity has been associated with several immunodeficiency disorders including AIDS and Wiskott-Aldrich syndrome, and CD73 is expressed on a variety of lymphomas and leukaemias, including CLL and ALL (Zhao et al. 2011)

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 10% Glycerol
Immunogen
Recombinant human CD73 (amino acids 27-252) purified from E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse FO myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P21589 Related reagents
Entrez Gene
NT5E Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000166 nucleotide binding
GO:0005624 membrane fraction
GO:0005737 cytoplasm
GO:0006144 purine base metabolic process
GO:0006206 pyrimidine base metabolic process
GO:0006259 DNA metabolic process
GO:0008253 5'-nucleotidase activity
GO:0031225 anchored to membrane
GO:0046135 pyrimidine nucleoside catabolic process
Regulatory
For research purposes only

Applications of CD73 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA4622GA detects a band of approximately 63 kDa in human mesenchymal stem cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for CD73 antibody

  1. Ciccocioppo, R. et al. (2011) Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease.
    Gut. 60 (6): 788-98.
  2. Fausther, M. et al. (2014) NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.
    PLoS One. 9 (6): e98568.
  3. Asti, A. et al. (2010) Stem Cells Grown in Osteogenic Medium on PLGA, PLGA/HA, and Titanium Scaffolds for Surgical Applications.
    Bioinorg Chem Appl. : 831031.