CD71 antibody | DF1513

Mouse anti Human CD71

Product Type
Monoclonal Antibody
Clone
DF1513
Isotype
IgG1
Specificity
CD71

Product Code Applications Pack Size List Price Your Price Qty
MCA1148
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.2 mg loader
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loader
MCA1148T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 µg loader
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loader
MCA1148GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a ~190 kDa homodimeric glycoprotein expressed by proliferating cells. CD71 is also known as the transferrin receptor. Mutation of the TFRC gene has been implicated in the development of Immunodeficiency 46 (IMD46) a combined immunodeficiency characterized by early onset chronic diarrhea, recurrent infections and intermittent neutropenia and thrombocytopenia (Jabara et al. 2016)

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Mustelid
Ferret
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1148, MCA1148T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
MCA1148GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
KGI cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/25
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD71 antibody

  1. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  2. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  3. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6(3):e17454.
  4. Stockwin, L.H. et al. (2009) Artemisinin dimer anticancer activity correlates with heme-catalyzed reactive oxygen species generation and endoplasmic reticulum stress induction.
    Int J Cancer. 125: 1266-75.
  5. Janes, P.W. et al. (1999) Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.
    J Cell Biol. 147: 447-61.
  6. Makoveichuk, E. et al. (2012) Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed.
    Biochem Biophys Res Commun. 425:138-43.
  7. Procaccini, C. et al. (2012) Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses.
    J Immunol. 189: 2941-53.
  8. Weissgerber, P. et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
    FEMS Microbiol Lett. 219 (2): 173-9.
  9. View The Latest Product References
  10. Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
    Nat Commun. 8: 14750.

Flow Cytometry

Synonyms
Transferrin Receptor
RRID
AB_1125283
UniProt
P02786
Entrez Gene
TFRC
GO Terms
GO:0008233 peptidase activity
GO:0004998 transferrin receptor activity
GO:0005887 integral to plasma membrane
GO:0005768 endosome
GO:0005905 coated pit
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0033572 transferrin transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0055085 transmembrane transport
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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