CD71 antibody | 23D10
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Mouse anti Human CD71 (Soluble) antibody, clone 23D10 recognizes human soluble CD71 (also known as transferrin receptor, or TfR). CD71 is a homodimeric membrane protein, approximately 90 kDa in size. It is responsible for the uptake of iron into the cell, by binding to and internalising iron-bound transferrin (ferrotransferrin). After the iron is released into the cell, the apotransferrin is relocated to the cell surface and released into the circulation. When CD71 is cleaved at Arg-100, a soluble (or serum) form of the protein is released. The level of soluble CD71 is used to detect erythropoietin misuse by athletes. It is also used to diagnose iron deficiency anemia.
Mouse anti Human CD71 (Soluble) antibody, clone 23D10 recognizes human soluble transferrin receptor and the complex between soluble transferrin receptor and transferrin.
Mouse anti Human CD71 (Soluble) antibody, clone 23D10 recognizes human soluble transferrin receptor and the complex between soluble transferrin receptor and transferrin.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified human soluble transferrin receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P02786 Related reagents
- Entrez Gene
- TFRC Related reagents
- GO Terms
- GO:0008233 peptidase activity
- GO:0004998 transferrin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005768 endosome
- GO:0005905 coated pit
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- GO:0033572 transferrin transport
- GO:0042470 melanosome
- GO:0044419 interspecies interaction between organisms
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of CD71 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting 1 |
- 1Clone 23D10 recognises human CD71 (soluble) under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
