CD71 antibody | 23D10

100% Secure

Mouse anti Human CD71 (soluble)

Product Type
Monoclonal Antibody
Clone
23D10
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2775 E WB* 0.2 mg
Mouse anti Human CD71 (Soluble) antibody, clone 23D10 recognizes human soluble CD71 (also known as transferrin receptor, or TfR). CD71 is a homodimeric membrane protein, approximately 90 kDa in size. It is responsible for the uptake of iron into the cell, by binding to and internalising iron-bound transferrin (ferrotransferrin). After the iron is released into the cell, the apotransferrin is relocated to the cell surface and released into the circulation. When CD71 is cleaved at Arg-100, a soluble (or serum) form of the protein is released. The level of soluble CD71 is used to detect erythropoietin misuse by athletes. It is also used to diagnose iron deficiency anemia.

Mouse anti Human CD71 (Soluble) antibody, clone 23D10 recognizes human soluble transferrin receptor and the complex between soluble transferrin receptor and transferrin.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified human soluble transferrin receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02786 Related reagents
Entrez Gene
TFRC Related reagents
GO Terms
GO:0008233 peptidase activity
GO:0004998 transferrin receptor activity
GO:0005887 integral to plasma membrane
GO:0005768 endosome
GO:0005905 coated pit
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0033572 transferrin transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of CD71 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1
  1. 1Clone 23D10 recognises human CD71 (soluble) under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Applications

These CD71 antibodies can be used for applications including:


New interactive tool: immune cell and marker protein facts at your fingertips

All you need to know about immune cells, lineage, marker expression and the marker protein itself. Providing a direct link to antibodies available to your chosen marker.