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CD68 antibody | Y1/82A

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Anti Human CD68 Antibody, clone Y1/82A thumbnail image 1 Anti Human CD68 Antibody, clone Y1/82A thumbnail image 2 Anti Human CD68 Antibody, clone Y1/82A thumbnail image 3
Anti Human CD68 Antibody, clone Y1/82A gallery image 1
Figure A. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA6014A647). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD68 Antibody, clone Y1/82A gallery image 2
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse anti Human CD68 (MCA6014F). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD68 Antibody, clone Y1/82A gallery image 3
Figure A. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse anti Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD68:RPE

Product Type
Monoclonal Antibody
Clone
Y1/82A
Isotype
IgG2b
Specificity
CD68

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Product Code Applications Pack Size List Price Your Price Qty
MCA6014PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests loader
List Price Your Price
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Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Phytohaemagglutinin stimulated peripheral blood mononuclear cells.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the murine NS1 myeloma.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests
>
List Price Your Price
Description Mouse IgG2b Negative Control:RPE

Source Reference

  1. Davey, F.R. et al. (1988) Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages.
    J Clin Pathol. 41 (7): 753-8.

References for CD68 antibody

  1. Micklem, K. et al. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
    Br J Haematol. 73 (1): 6-11.
  2. Pulford, K.A. et al. (1990) Distribution of the CD68 macrophage/myeloid associated antigen.
    Int Immunol. 2 (10): 973-80.
  3. Holness, C.L. & Simmons, D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.
    Blood. 81 (6): 1607-13.
  4. Mahida, Y.R. et al. (1997) Migration of human intestinal lamina propria lymphocytes, macrophages and eosinophils following the loss of surface epithelial cells.
    Clin Exp Immunol. 109 (2): 377-86.

UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane

MCA6014PE

INN1705

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