CD65s antibody | VIM-2
Mouse anti Human CD65s antibody, clone VIM-2 selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.
CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.
Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansen et al. 1992).
CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.
Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansen et al. 1992).
Product Details
- Target Species
- Human
- Product Form
- Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- THP1 (human acute monocytic leukaemia cells).
- Approx. Protein Concentrations
- IgM concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD65s antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control:FITC | MCA692F | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD65s antibody
-
Gooi, H.C. et al. (1985) Differences in the fine specificities of monoclonal (Class A) antibodies to human myeloid cells.
Clin Exp Immunol. 60 (1): 151-8. -
Lund-johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65).
J Immunol. 148 (10): 3221-9. -
Knapp, W. et al. (1994) Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis.
Cytometry. 18 (4): 187-98. -
Lund-johansen, F. et al. (1990) Flow cytometric assay for the measurement of human bone marrow phenotype, function and cell cycle.
Cytometry. 11 (5): 610-6. -
Bengtson, P. et al. (2002) Polymorphonuclear Leukocytes from Individuals Carrying the G329A Mutation in the α1,3-Fucosyltransferase VII Gene (FUT7) Roll on E- and P-Selectins
J Immunol. 169: 3940-6. -
Buffone, A. et al. (2013) Silencing α1,3-fucosyltransferases in human leukocytes reveals a role for FUT9 during E-selectin mediated cell adhesion.
J Biol Chem. 288: 1620-33. -
Nakayama, F. et al. (2001) CD15 expression in mature granulocytes is determined by alpha 1,3-fucosyltransferase IX, but in promyelocytes and monocytes by alpha 1,3-fucosyltransferase IV.
J Biol Chem. 276: 16100-6. -
Rao, R.M. et al. (2001) The S128R polymorphism of E-selectin mediates neuraminidase-resistant tethering of myeloid cells under shear flow.
Eur J Immunol. 32: 251-60. -
Paietta, E. et al. (2003) Low expression of the myeloid differentiation antigen CD65s, a feature of poorly differentiated AML in older adults: study of 711 patients enrolled in ECOG trials.
Leukemia. 17: 1544-50. -
Bengtson, P. et al. (2001) Identification of a missense mutation (G329A;Arg(110)--> GLN) in the human FUT7 gene.
J Biol Chem. 276: 31575-82. -
Oehler, L. et al. (1998) Neutrophil granulocyte-committed cells can be driven to acquire dendritic cell characteristics.
J Exp Med. 187: 1019-28.
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