CD64 antibody | 10.1
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|Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI.
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Baboon Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- Human monocytes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or email@example.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
References for CD64 antibody
Dougherty, G.J. et al. (1987) The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.
Eur J Immunol. 17 (10): 1453-9.
Beekman, J.M. et al. (2004) Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.
Proc Natl Acad Sci U S A.101: 10392-7.
Tanaka, M. et al. (2009) Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.
J Immunol. 183: 2349-55.
Fet, N.G. et al. (2012) Reduction of activated macrophages after ischaemia-reperfusion injury diminishes oxidative stress and ameliorates renal damage.
Nephrol Dial Transplant. 27 (8): 3149-55.
Wagner, C. et al. (2008) T lymphocytes in acute bacterial infection: increased prevalence of CD11b(+) cells in the peripheral blood and recruitment to the infected site.
Immunology. 125: 503-9.
Eisenhardt, S.U. et al. (2009) Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.
Circ Res. 105: 128-37.
Fadlon,. E. et al. (1998) Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64.
Blood. 91: 266-74.
Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73.
View The Latest Product References
Navarro-López, F. et al. (2003) Late T-lymphocyte and monocyte activation in coronary restenosis. Evidence for a persistent inflammatory/immune mechanism?
Rev Esp Cardiol. 56: 465-72.
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8.
Kapelski S et al. (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum.
J Leukoc Biol. 96 (6): 1131-42.
Loi, A.L.T. et al. (2017) Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Respir Res. 18 (1): 100.
Hristodorov, D. et al. (2016) Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages.
Immunol Cell Biol. 94 (5): 470-8.
Kahn, F. et al. (2008) Antibodies against a surface protein of
Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149.
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110.
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0006911 phagocytosis, engulfment
- GO:0019864 IgG binding
- GO:0045087 innate immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
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