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CD62P antibody | AK-6

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Anti Human CD62P Antibody, clone AK-6 thumbnail image 1 Anti Human CD62P Antibody, clone AK-6 thumbnail image 2 Anti Human CD62P Antibody, clone AK-6 thumbnail image 3 Anti Human CD62P Antibody, clone AK-6 thumbnail image 4 Anti Human CD62P Antibody, clone AK-6 thumbnail image 5
Anti Human CD62P Antibody, clone AK-6 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse anti Human CD62P (MCA796A700). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD62P Antibody, clone AK-6 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Pacific Blue conjugated Mouse IgG1 isotype control (MCA928PB). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Pacific Blue conjugated Mouse anti Human CD62P (MCA796PB). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD62P Antibody, clone AK-6 gallery image 3
Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (MCA796GA)
Anti Human CD62P Antibody, clone AK-6 gallery image 4
Formalin fixed, paraffin embedded human tonsil vessel stained with Mouse Anti-Human CD62P antibody, clone AK-6 (MCA796GA) followed by biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.
Anti Human CD62P Antibody, clone AK-6 gallery image 5
Published customer image:
FITC conjugated Mouse anti Human CD62P antibody, clone AK-6 (MCA796F) used to assess activationof CD41 positive platelets by flow cytometry.
Image caption:
Dot plot: Log SSC (Side Scatter) versus Log FL2 (Filter 2) (in orange). A gate was drawn around CD41+ events, delineating the platelet population. FL1 Histogram: count versus log FL1 (in green). The gate CD41+ was applied to analyze FL1. ‘%R’ represents the percentage of CD-62p positive events. (A) Upper panels, TRAP-activated platelets (TRAP Ctl+); lower panels, resting platelets (PBS Ctl−). A total of 10,000 platelets (CD41+ events) were analyzed in each run and the percentage of activated platelets (CD62p positive events) was evaluated (FL1 histogram). For each series, a cursor indicating the activation threshold was placed at the intersection of the FL1 histograms of the negative control (PBS Ctl−) and the positive control (TRAP Ctl+) and the cut-off was determined. In this example, with resting platelets (PBS Ctl−), we observed 11.7% CD62p-positive events corresponding to spontaneous platelet activation and electronic noise. After platelet activation with TRAP (TRAP Ctl+), we observed 86.5% CD62p-positive events corresponding to maximal platelet activation. (B) Left panels: Platelets activated with a highly HIT positive plasma. We observed 88.1% CD62p-positive events with 0.3 IU/mL. UFH (upper panel) and 12.9% CD62p-positive events with 100 IU/mL UFH (lower panel). The HEPLA index was 100% Middle panels: Platelets activated with intermediately HIT positive plasma. We observed 53.4% CD62p-positive events with 0.3 IU/mL UFH (upper panel) and 18.4% CD62p-positive events with 100 IU/mL UFH (lower panel). The HEPLA index was 47%. Right panels: Platelets incubated with HIT negative plasma. We observed 13.5% CD62p-positive events with 0.3 IU/mL UFH (upper panel) and 12.5% with 100 IU/mL UFH (lower panel). The HEPLA index was 1%. TRAP: Thrombin Receptor Agonist Peptide, PBS: Phosphate-buffered saline, HIT: Heparin-induced thrombocytopenia, UFH: Unfractioned heparin, HEPLA: Heparin Platelet Activation.

From: Tardy-Poncet, B.; Montmartin, A.; Piot, M.; Alhenc-Gelas, M.; Nguyen, P.; Elalamy, I.; Greinacher, A.; Maistre, E.D.; Lasne, D.; Horellou, M.-H.; Le Gal, G.; Lecompte, T.; Tardy, B.; on behalf of the GFHT-HIT Study Group.
Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice.
Biomedicines 2021, 9, 332.
doi: 10.3390/biomedicines9040332
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Human CD62P:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
AK-6
Isotype
IgG1
Specificity
CD62P

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MCA796A700
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Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell

CD62P plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the platelet alpha granule and is rapidly translocated to the plasma membrane upon activation (Stenberg et al. 1985).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human platelet membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader
List Price Your Price
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Description Mouse IgG1 Negative Control:Alexa Fluor® 700

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD62P antibody

  1. Dunlop, L.C. et al. (1992) Characterization of GMP-140 (P-selectin) as a circulating plasma protein.
    J Exp Med. 175 (4): 1147-50.
  2. Skinner, M.P. et al. (1989) Characterization of human platelet GMP-140 as a heparin-binding protein.
    Biochem Biophys Res Commun. 164 (3): 1373-9.
  3. Skinner, M.P. et al. (1991) GMP-140 binding to neutrophils is inhibited by sulfated glycans.
    J Biol Chem. 266 (9): 5371-4.
  4. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  5. Bevilacqua, M.P. & Nelson, R.M. (1993) Selectins.
    J Clin Invest. 91 (2): 379-87.
  6. Roos-Engstrand, E. et al. (2005) Increased expression of p38 MAPK in human bronchial epithelium after lipopolysaccharide exposure.
    Eur Respir J. 25 (5): 797-803.
  7. Kornerup, K.N. et al. (2010) Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration.
    J Appl Physiol. 109: 758-67.
  8. Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
    FASEB J. 22: 682-90.
    9. View The Latest Product References
  9. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  10. Wassmer, S.C. at al. (2008) Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria-possible modulation in vivo by thrombocytopenia.
    J Infect Dis. 197: 72-8.
  11. Theoret, J.F. et al. (2001) P-selectin antagonism with recombinant p-selectin glycoprotein ligand-1 (rPSGL-Ig) inhibits circulating activated platelet binding to neutrophils induced by damaged arterial surfaces.
    J Pharmacol Exp Ther. 298: 658-64
  12. Turner, C.P. et al. (2003) The role of P-selectin in the immune destruction of platelets.
    Br J Haematol. 121: 623-31.
  13. van Nispen tot Pannerden, H. et al. (2010) The platelet interior revisited: electron tomography reveals tubular alpha-granule subtypes.
    Blood. 116: 1147-56.
  14. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
    Blood. 116 (12): 2183-91.
  15. Kitaya, K. & Yasuo, T. (2010) Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis.
    Mod Pathol. 23 (8): 1136-46.
  16. Xiong, G.M. et al. (2015) Imparting electroactivity to polycaprolactone fibers with heparin-doped polypyrrole: Modulation of hemocompatibility and inflammatory responses.
    Acta Biomater. 23: 240-9.
  17. Liao, Y. et al. (2017) Tailoring of TiO2 films by H2SO4 treatment and UV irradiation to improve anticoagulant ability and endothelial cell compatibility.
    Colloids Surf B Biointerfaces. 155: 314-22.
  18. Christersson, C. et al. (2013) Evaluation of microparticles in whole blood by multicolour flow cytometry assay.
    Scand J Clin Lab Invest. 73(3): 229-39.
  19. Tardy-Poncet, B. et al. (2021) Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice
    Biomedicines. 9 (4): 332.
  20. Cipok, M. et al. (2019) Pathogenic heparin-induced thrombocytopenia and thrombosis (HIT) antibodies determined by rapid functional flow cytometry.
    Eur J Haematol. 103 (3): 225-233.
  21. Jiang, T. et al. (2019) Hyaluronic Acid Nanoparticle Composite Films Confer Favorable Time-Dependent Biofunctions for Vascular Wound Healing.
    ACS Biomater Sci Eng. 5 (4): 1833-48.

Flow Cytometry

Immunofluorescence

Synonyms
P-SELECTIN
UniProt
P16109
Entrez Gene
SELP
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
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