CD62P antibody | AK-6

Mouse anti Human CD62P:Alexa Fluor® 700

Product Type
Monoclonal Antibody

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell

CD62P plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the platelet alpha granule and is rapidly translocated to the plasma membrane upon activation (Stenberg et al. 1985).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Human platelet membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG1 Negative Control:Alexa Fluor® 700

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD62P antibody

  1. Dunlop, L.C. et al. (1992) Characterization of GMP-140 (P-selectin) as a circulating plasma protein.
    J Exp Med. 175 (4): 1147-50.
  2. Skinner, M.P. et al. (1989) Characterization of human platelet GMP-140 as a heparin-binding protein.
    Biochem Biophys Res Commun. 164 (3): 1373-9.
  3. Skinner, M.P. et al. (1991) GMP-140 binding to neutrophils is inhibited by sulfated glycans.
    J Biol Chem. 266 (9): 5371-4.
  4. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  5. Bevilacqua, M.P. & Nelson, R.M. (1993) Selectins.
    J Clin Invest. 91 (2): 379-87.
  6. Roos-Engstrand, E. et al. (2005) Increased expression of p38 MAPK in human bronchial epithelium after lipopolysaccharide exposure.
    Eur Respir J. 25 (5): 797-803.
  7. Kornerup, K.N. et al. (2010) Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration.
    J Appl Physiol. 109: 758-67.
  8. Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
    FASEB J. 22: 682-90.
  9. View The Latest Product References
  10. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  11. Wassmer, S.C. at al. (2008) Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria-possible modulation in vivo by thrombocytopenia.
    J Infect Dis. 197: 72-8.
  12. Theoret, J.F. et al. (2001) P-selectin antagonism with recombinant p-selectin glycoprotein ligand-1 (rPSGL-Ig) inhibits circulating activated platelet binding to neutrophils induced by damaged arterial surfaces.
    J Pharmacol Exp Ther. 298: 658-64
  13. Turner, C.P. et al. (2003) The role of P-selectin in the immune destruction of platelets.
    Br J Haematol. 121: 623-31.
  14. van Nispen tot Pannerden, H. et al. (2010) The platelet interior revisited: electron tomography reveals tubular alpha-granule subtypes.
    Blood. 116: 1147-56.
  15. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
    Blood. 116 (12): 2183-91.
  16. Kitaya, K. & Yasuo, T. (2010) Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis.
    Mod Pathol. 23 (8): 1136-46.
  17. Xiong, G.M. et al. (2015) Imparting electroactivity to polycaprolactone fibers with heparin-doped polypyrrole: Modulation of hemocompatibility and inflammatory responses.
    Acta Biomater. 23: 240-9.
  18. Liao, Y. et al. (2017) Tailoring of TiO2 films by H2SO4 treatment and UV irradiation to improve anticoagulant ability and endothelial cell compatibility.
    Colloids Surf B Biointerfaces. 155: 314-22.
  19. Christersson, C. et al. (2013) Evaluation of microparticles in whole blood by multicolour flow cytometry assay.
    Scand J Clin Lab Invest. 73(3): 229-39.
  20. Tardy-Poncet, B. et al. (2021) Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice
    Biomedicines. 9 (4): 332.
  21. Cipok, M. et al. (2019) Pathogenic heparin-induced thrombocytopenia and thrombosis (HIT) antibodies determined by rapid functional flow cytometry.
    Eur J Haematol. 103 (3): 225-233.
  22. Jiang, T. et al. (2019) Hyaluronic Acid Nanoparticle Composite Films Confer Favorable Time-Dependent Biofunctions for Vascular Wound Healing.
    ACS Biomater Sci Eng. 5 (4): 1833-48.

Flow Cytometry


Entrez Gene
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
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