CD60 antibody | M-T6004

100% Secure

Mouse anti Human CD60

Product Type
Monoclonal Antibody
Clone
M-T6004
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1314 C F IP 2 ml
Mouse anti Human CD60 antibody, clone M-T6004 recognizes the human CD60 antigen, a carbohydrate structure GD3 (Neu5Acα8Neu5Acα3Galβ4GlcCer) modified by a terminal 9-0-acetylated disialosyl group (9-O-acetyl GD3). Mouse anti human CD60 antibody clone M-T6004 does not recognize 7-O-acetyl GD3 or non-acetylated GD3. CD60 has been divided into 3 sub-types depending on the acetylation present. Non acetylated GD3 is termed CD60a and 7-O-acetyl GD3 termed CD60c. 9-O-acetyl GD3 as recognized by clone M-T6004 has been designated CD60b.

CD60b is present on CD8+ve helper T cells (Rieber and Rank 1994), CD4+ve memory T cells and on activated B cells (Vater et al. 1997). Expression has also been reported on neuroectodermal cells (Carr et al. 1995) and in breast carcinomas (Gocht et al. 1998). CD60b is not expressed by naive T cells (Wada et al. 1998). GD3 has been described as a pro-apoptotic messenger targeting mitochondria in many lymphoid and myeloid cell types (García-Ruiz et al. 2000). Acetylation abrogates the pro-apoptotic activity of GD3 (Malisan et al. 2002) and CD60b protects tumor cells from apoptosis (Kniep et al. 2006).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rainbow Trout
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by gel filtration from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Purified CD60 antigen from PBL; 9-O-acetyl-GD3 from buttermilk
Approx. Protein Concentrations
IgM concentration 100ug/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the P3. X63.Ag.8653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD60 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgM Negative Control MCA692 100 Tests F

Product Specific References

References for CD60 antibody

  1. Rieber, E.P. & Rank, G. (1994) CDw60: a marker for human CD8+ T helper cells.
    J Exp Med. 179 (4): 1385-90.
  2. Saalmuller, A. et al. (2007) Summary of the animal homologue section of HLDA8.
    Vet Immunol Immunopathol. 119: 2-13.
  3. Malisan, F. et al. (2002) Acetylation suppresses the proapoptotic activity of GD3 ganglioside.
    J Exp Med. 196: 1535-41.
  4. Kniep, B. et al. (2006) 9-O-acetyl GD3 protects tumor cells from apoptosis.
    Int J Cancer. 119: 67-73.
  5. Erdmann, M. et al. (2006) Differential surface expression and possible function of 9-O- and 7-O-acetylated GD3 (CD60 b and c) during activation and apoptosis of human tonsillar B and T lymphocytes.
    Glycoconj J. 23: 627-38.
  6. Fischer, U. and Koellner, B. (2007) Cross-reactivity of human leukocyte differentiation antigen monoclonal antibodies on carp and rainbow trout cells.
    Vet Immunol Immunopathol. 119: 142-55.
  7. Mukherjee, K. et al. (2008) O-acetylation of GD3 prevents its apoptotic effect and promotes survival of lymphoblasts in childhood acute lymphoblastic leukaemia
    J Cell Biochem. 105: 724-34.
  8. Schauer, R. et al. (2011) O-Acetylated sialic acids and their role in immune defense.
    Adv Exp Med Biol. 705: 525-48.
  9. Belov, L. et al. (2017) Surface Profiling of Extracellular Vesicles from Plasma or Ascites Fluid Using DotScan Antibody Microarrays.
    Methods Mol Biol. 1619: 263-301.