CD58 antibody | 2D11-B10

Mouse anti Human CD58:Preservative Free

Product Type
Monoclonal Antibody
Clone
2D11-B10
Isotype
IgG2a
Specificity
CD58

Product Code Applications Pack Size List Price Your Price Qty
MCA4000Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD58 antibody, clone 2D11-B10 recognizes human CD58, also known as Lymphocyte function-associated antigen 3 or LFA-3. CD58 is a 250 amino acid ~29 kDa single pass type-1 membrane glycoprotein and ligand for CD2. Multiple isoforms of CD58 can be produced by alternative splicing, Mouse anti Human CD58 antibody, clone 2D11-B10 recognizes an epitope common to all forms of CD58.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1 - 241 of human CD58.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Further Reading

  1. Wingren, A.G. et al. (2017) T Cell Activation Pathways: B7, LFA-3, and ICAM-1 Shape Unique T Cell Profiles.
    Crit Rev Immunol. 37 (2-6): 463-81.

Synonyms
LFA-3
RRID
AB_2076104
UniProt
P19256
Entrez Gene
CD58
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016337 cell-cell adhesion
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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