CD58 antibody | 2D11-B10
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P IF WB ResetMouse anti Human CD58:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2D11-B10
- Isotype
- IgG2a
- Specificity
- CD58
Antibody Quality and Performance Guarantee
| Mouse anti Human CD58 antibody, clone 2D11-B10 recognizes human CD58, also known as Lymphocyte function-associated antigen 3 or LFA-3. CD58 is a 250 amino acid ~29 kDa single pass type-1 membrane glycoprotein and ligand for CD2. Multiple isoforms of CD58 can be produced by alternative splicing, Mouse anti Human CD58 antibody, clone 2D11-B10 recognizes an epitope common to all forms of CD58. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1 - 241 of human CD58.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Immunohistology - Paraffin 1 | |
0.1 | 10 ug/ml |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Wingren, A.G. et al. (2017) T Cell Activation Pathways: B7, LFA-3, and ICAM-1 Shape Unique T Cell Profiles.
Crit Rev Immunol. 37 (2-6): 463-81.
- Synonyms
- LFA-3
- RRID
- AB_2076104
- UniProt
- P19256
- Entrez Gene
- CD58
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0005887 integral to plasma membrane
- GO:0031225 anchored to membrane
- GO:0016337 cell-cell adhesion
- GO:0050900 leukocyte migration
MCA4000Z
160229
160715
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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