CD56 antibody | 123C3

Mouse anti Human CD56:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
123C3
Isotype
IgG1
Specificity
CD56

Product Code Applications Pack Size List Price Your Price Qty
MCA2693A700
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Mouse anti Human CD56 antibody, clone 123C3 recognizes human neural cell adhesion molecule (NCAM), otherwise known as CD56. The CD56 molecule is a cell surface glycoprotein expressed on neuroendocrine cells, natural killer cells and a subset of T cells in the peripheral blood. Three main isoforms of CD56 exist. Neurons express the largest 180 kDa form, while hemopoietic cells express the 140 kDa isoform. Mouse anti Human CD56 antibody, clone 123C3 recognizes both the 140 kDa and the 180 kDa isoform of the CD56 protein.

In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion and is implicated in neural development. CD56 is also expressed on thyroid follicular epithelium and may play a role in autoimmune disease of the thyroid. CD56 is expressed in a range of tumors including tumors of the lung, neural derived malignancies and natural killer cell lymphomas.

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Membrane preparation of small lung carcinoma.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the murine Sp2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul lysed whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:Alexa Fluor® 700

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD56 antibody

  1. Schol, D.J. et al. (1988) Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung tumours, recognizes mainly, but not exclusively, endocrine and neuron-supporting normal tissues.
    Int J Cancer Suppl. 2: 34-40.
  2. Mooi, W.J. et al. (1988) Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358 resected lung tumours.
    Mol Cell Probes. 2 (1): 31-7.
  3. Moolenaar, C.E. et al. (1990) Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212.
    Cancer Res. 50 (4): 1102-6.
  4. Aloysius, M.M. et al. (2010) Mucins and CD56 as markers of tumour invasion and prognosis in periampullary cancer.
    Br J Surg. 97: 1269-78
  5. Wanka, G. et al. (2020) LDOC1 as Negative Prognostic Marker for Vulvar Cancer Patients.
    Int J Mol Sci. 21 (23) Dec 05 [Epub ahead of print].
  6. Heylmann, D. et al. (2021) Comparison of DNA repair and radiosensitivity of different blood cell populations.
    Sci Rep. 11 (1): 2478.

Synonyms
N-CAM
RRID
AB_915199
UniProt
P13591
Entrez Gene
NCAM1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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