Mouse anti Human CD50 antibody, clone Bu65 recognizes human CD50, also known as Inter-cellular adhesion molecule-3 (ICAM-3). CD50 is a ~110 kDa type I transmembrane glycoprotein, expressed mainly on resting leukocytes. It interacts with lymphocyte function-associated antigen-1 (LFA-1) and DC-SIGN, and is involved in the intercellular adhesion of leukocytes and augments CD3 signalling in T-lymphocyte activation.
CD50 expression induces cancer cell proliferation and over-expression may contribute to cancer progression. It has also been implicated in limiting HIV-1 replication.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
regulation of immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD50 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
Biggins, J.E. et al. (2007) ICAM-3 influences human immunodeficiency virus type 1 replication in CD4(+) T cells independent of DC-SIGN-mediated transmission.
Virology. 364 (2): 383-94.
Berney, S.M. et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes.
J Leukoc Biol. 65 (6): 867-74.
Kim, Y.G. et al. (2006) ICAM-3-induced cancer cell proliferation through the PI3K/Akt pathway.
Cancer Lett. 239 (1): 103-10.