CD50 antibody | Bu65
Mouse anti Human CD50 antibody, clone Bu65 recognizes human CD50, also known as Inter-cellular adhesion molecule-3 (ICAM-3). CD50 is a ~110 kDa type I transmembrane glycoprotein, expressed mainly on resting leukocytes. It interacts with lymphocyte function-associated antigen-1 (LFA-1) and DC-SIGN, and is involved in the intercellular adhesion of leukocytes and augments CD3 signalling in T-lymphocyte activation.
CD50 expression induces cancer cell proliferation and over-expression may contribute to cancer progression. It has also been implicated in limiting HIV-1 replication.
CD50 expression induces cancer cell proliferation and over-expression may contribute to cancer progression. It has also been implicated in limiting HIV-1 replication.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P32942 Related reagents
- Entrez Gene
- ICAM3 Related reagents
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0016337 cell-cell adhesion
- GO:0050776 regulation of immune response
- Regulatory
- For research purposes only
Applications of CD50 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/200 | |
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
Further Reading
-
Biggins, J.E. et al. (2007) ICAM-3 influences human immunodeficiency virus type 1 replication in CD4(+) T cells independent of DC-SIGN-mediated transmission.
Virology. 364 (2): 383-94. -
Berney, S.M. et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes.
J Leukoc Biol. 65 (6): 867-74. -
Kim, Y.G. et al. (2006) ICAM-3-induced cancer cell proliferation through the PI3K/Akt pathway.
Cancer Lett. 239 (1): 103-10.
