CD5 antibody | MF7-14.5

Mouse anti Human CD5:RPE

Product Type
Monoclonal Antibody
Clone
MF7-14.5
Isotype
IgG2a
Specificity
CD5

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MCA1341PE
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Mouse anti Human CD5 antibody, clone MF7-14.5 recognizes the CD5 cell surface glycoprotein, a ~67 kDa antigen expressed by T lymphocytes and by a subset of B lymphocytes.

CD5 is also expressed by tumor cells in cases of B-cell Chronic Lymphocytic Leukemia (B-CLL).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul washed

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader
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Description Mouse IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD5 antibody

  1. Deka, C. et al. (2012) Lymphocyte analysis for monitoring the progression of immunodeficiency virus
    Patent Application US20120264109 A1

RRID
AB_321385
UniProt
P06127
Entrez Gene
CD5
GO Terms
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
GO:0008037 cell recognition
GO:0008283 cell proliferation
View more products with CD5 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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