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CD49f antibody | NKI-GoH3

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Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 1 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 2 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 3 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 4 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 5 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 6 Anti Human CD49f Antibody, clone NKI-GoH3 thumbnail image 7
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 1
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Rat IgG2a isotype control (MCA6005F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and FITC conjugated Rat anti Human CD49f (MCA699F). All experiments performed on human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 2
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and RPE conjugated Rat IgG2a isotype control (MCA6005PE). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and RPE conjugated Rat anti Human CD49F (MCA699PE). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 3
Published customer image:
Rat anti Human CD49f antibody, clone NKI-GoH3 (MCA699) used for the demonstration of ItgA6 in basement keratinocytes by immunofluorescence.
Image caption:
Blocking Itgb1 activity ameliorates the chronic hyperproliferative dermatitis phenotype in Sharpincpdm/cpdm mice.
D) Representative frozen skin sections from Sharpin+/? and Sharpincpdm/cpdm mice treated with PBS (control) or anti-Itgb1, stained for Krt14 (C) or proliferation (Ki67) and basal keratinocytes (Itga6) (D). Scale bars are 20 μm.

From: Peuhu E, Salomaa SI, De Franceschi N, Potter CS, Sundberg JP, Pouwels J (2017)
Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice.
PLoS ONE12(10): e0186628.
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 4
Published customer image:
Rat anti Human CD49f antibody, clone NKI-GoH3 (MCA699) used for immuno-phenotyping and evaluation of integrin α6 expression on cultured myofibres by immunofluorescence.
Image caption:
Integrin-α6 mediates laminin-α1 control of SC expansion. a Immunofluorescence analysis of cultured EDL myofibers showing integrin-α6 upregulation in activated SCs. Inserts show high magnification images of satellite cells indicated by the white arrows. Top panels show images in the green channel only, bottom panels show the merge images, inserts are higher magnification of merge image. Scale bar, 50 μm. b Quantification of integrin-α6 expression in satellite cells from cultured myofibers. n = 3 with 20–30 fibers per time point. c Integrin-α6 (red) distribution in 2 dpi and 4 dpi injured TA muscle of Tg(Pax7-GFP) mice. Images of the blue/red channels and green/red channels show colocalization of integrin-α6 with satellite cells (white arrows) and with predicted endothelial cells (white arrowheads). Scale bar, 20 μm. d Immuno-colocalization of integrin-α6 (red) with either MyoD (green, activated satellite cell marker), F4/80 (green, pan macrophage marker), CD206 (green, M2 macrophage marker), or CD31 (green, endothelial cell marker) following cardiotoxin-mediated injury of TA muscles. Inserts are high magnification images of areas delineated by a dotted white line. Note that integrin-α6 is primarily associated with endothelial cells at 2 dpi, whereas it is primarily detected at the surface of macrophages (M1 and M2) and satellite cells at 4 dpi. By 7 dpi, integrin-α6 returns to being restricted to endothelial cells. Scale bar, 50 μm. Graph shows mean + sem.

From: Rayagiri SS, Ranaldi D, Raven A, Mohamad Azhar NIF, Lefebvre O, Zammit PS, Borycki AG.
Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
Nat Commun. 2018 Mar 14;9(1):1075.
Published under a Creative commons CC BY license.
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 5
Published customer image:
Low endotoxin Rat anti Human CD49f antibody, clone NKI-GoH3 (MCA699EL) used in the culture of myofibres in vitro for subsequent immuno-phenotyping by immunofluorescence.
Image caption:
Laminin-111 treatment increases SC proliferation and self-renewal. a Representative images of myofibers cultured in control conditions or in the presence of laminin-111 or Cd49f, and analyzed by immunofluorescence for Pax7 (red), Myf5 (green), MyoD (green), Caveolin-1 (green), and Myogenin (red). Scale bar: 50 μm. b Quantification of satellite cell populations in myofiber cultures supplemented with Laminin-111, Cd49f, or both. Graph shows mean + sem. *P <0.05, **P <0.01, ***P <0.001 (t-test)

From: Rayagiri SS, Ranaldi D, Raven A, Mohamad Azhar NIF, Lefebvre O, Zammit PS, Borycki AG.
Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
Nat Commun. 2018 Mar 14;9(1):1075.
Published under a Creative commons CC BY license.
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 6
Published customer image:
Rat anti Human CD49f antibody, clone NKI-GoH3 (MCA699) used to stain cultured mouse cells by immunofluorescence.
Image caption:
Immunostaining of CD49f cell surface marker and laminin. Fluorescent immunocytochemistry was performed on sub-cultured CD49f+ cells with Tween-20 (upper row; for both cytoplasm and cell surface) or without (lower row; for cell surface) on the same sections. The CD49f marker was stained red in (c) and (g); laminin was stained green in (d) and (h); nuclei were stained blue with DAPI in (b) and (f). Overlaid images of 2 sets of 3 images are colored yellow in (a) and (e). The experiment was performed using sub-cultured CD49f+ cells fractionated from the salivary glands of 3 mice, and 3 independent experiments were carried out, and a typical set of images is shown. Scale bar: 10 μm.

From: Ikeda A, Yamamoto T, Mineshiba J, Takashiba S.
Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells.
Sci Rep. 2020 Nov 17;10(1):19959.
doi: 10.1038/s41598-020-77004-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD49f Antibody, clone NKI-GoH3 gallery image 7
Published customer image:
Phycoerythrin conjugated Rat anti Human CD49f antibody, clone NKI-GoH3 (MCA699PE) used to identify epidermal cell populations from mice by flow cytometry.
Image caption:
Representative flow cytometry dot plots from mice of indicated genotypes (top) and ages (right) showing the different epidermal cell populations according to CD34 and integrin α6 surface expression. The basal bulge population abundance is indicated (n = 6 per genotype and age).

From: Lorenzo-Martín LF, Menacho-Márquez M, Fernández-Parejo N, Rodríguez-Fdez S, Pascual G, Abad A, Crespo P, Dosil M, Benitah SA, Bustelo XR.
The Rho guanosine nucleotide exchange factors Vav2 and Vav3 modulate epidermal stem cell function.
Oncogene. 2022 May 9 [Epub ahead of print].
doi: 10.1038/s41388-022-02341-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Rat anti Human CD49f:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
NKI-GoH3
Isotype
IgG2a
Specificity
CD49f

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Product Code Applications Pack Size List Price Your Price Qty
MCA699A647
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49f, also known as the VLA-6 alpha chain. CD49f is a 1107 amino acid ~120 kDa cell surface glycoprotein that forms distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex expressed on various human epithelial cells.

Rat anti Human CD49f antibody, clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common acute lymphoblastic leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin.

Rat anti Human CD49f antibody, clone NKI-GoH3 blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog
Pig
Cynomolgus monkey
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
BALB/c mouse mammary tumor cells
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negitive/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA6005A647 F 100 Tests/1ml loader
List Price Your Price
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Description Rat IgG2a Negative Control:Alexa Fluor® 647

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD49f antibody

  1. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
    J Histochem Cytochem. 34 (8): 1037-46.
  2. Sonnenberg, A. et al. (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody.
    J Biol Chem. 262 (21): 10376-83.
  3. Sonnenberg, A. et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic.
    J Biol Chem. 263 (28): 14030-8.
  4. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6.
    Nature. 336 (6198): 487-9.
  5. Hemler, M.E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures.
    J Biol Chem. 263 (16): 7660-5.
  6. Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055.
    Oxford University Press.
  7. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens.
    Edited by Knapp, W. et al. Oxford University Press p1029-1032.
  8. Sonnenberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties.
    J Cell Sci. 96 ( Pt 2): 207-17.
    9. View The Latest Product References
  9. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8.
    J Cell Biol. 110 (6): 2145-55.
  10. Alais, S. et al. (2001) HEMCAM/CD146 downregulates cell surface expression of beta1 integrins.
    J Cell Sci. 114: 1847-59.
  11. Dangerfield, J. et al. (2002) PECAM-1 (CD31) homophilic interaction up-regulates alpha6beta1 on transmigrated neutrophils in vivo and plays a functional role in the ability of alpha6 integrins to mediate leukocyte migration through the perivascular basement membrane.
    J Exp Med. 196: 1201-11.
  12. Le Bellego, F. et al. (2005) Cytoskeleton reorganization mediates alpha6beta1 integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells.
    Reprod Biol Endocrinol. 3: 19.
  13. Anderson, C. et al. (2009) Sonic hedgehog-dependent synthesis of laminin alpha1 controls basement membrane assembly in the myotome.
    Development. 136: 3495-504.
  14. Jensen, K.B. et al. (2010) Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis.
    Nat Protoc. 5 (5): 898-911.
  15. da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
    Am J Pathol. 177: 1534-48.
  16. Collins, C.A. et al. (2011) Reprogramming adult dermis to a neonatal state through epidermal activation of β-catenin
    Development.138: 5189-99.
  17. Schäfer, G. et al. (2013) The role of inflammation in HPV infection of the Oesophagus.
    BMC Cancer. 13: 185.
  18. Moreira, M.L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
    Vet Parasitol. 220: 33-45.
  19. Mastrogiannaki, M. et al. (2016) β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.
    J Invest Dermatol. 136 (6): 1130-42.
  20. Haining, E.J. et al. (2017) Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.
    Platelets. 28 (7): 629-42.
  21. Peuhu. E, et al. (2017) Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice
    PLOS ONE. 12 (10): e0186628.
  22. Rayagiri, S.S. et al. (2018) Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal.
    Nat Commun. 9 (1): 1075.
  23. Loureiro, J. et al. (2019) Conjugation of the T1 sequence from CCN1 to fibrin hydrogels for therapeutic vascularization.
    Mater Sci Eng C Mater Biol Appl. 104: 109847.
  24. Ikeda, A. et al. (2020) Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells.
    Sci Rep. 10 (1): 19959.
  25. Lorenzo-Martín, L.F. et al. (2022) The Rho guanosine nucleotide exchange factors Vav2 and Vav3 modulate epidermal stem cell function.
    Oncogene. May 09 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Functional Assays

Immunofluorescence

Synonyms
Integrin Alpha 6 Chain
VLA-6
RRID
AB_324801
UniProt
P23229
Entrez Gene
ITGA6

MCA699A647

147966

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