Mouse anti Human CD49e antibody, clone JBS5
recognizes the Alpha 5 integrin, also known as CD49e, VLA-5, Fibronectin receptor subunit alpha or Integrin alpha-F. CD49e is a 1049 amino acid ~130kDa single pass type-1 transmembrane glycoprotein containing seven FG-GAP
repeats. Mouse anti human CD49e antibody, clone JBS5 been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines (Shen et al. 1991
). It also inhibits the adherence of cells to fibronectin.
Removal of sodium azide is recommended prior to use in functional assays.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
platelet-derived growth factor receptor binding
integrin-mediated signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
wound healing, spreading of epidermal cells
vascular endothelial growth factor receptor 2 binding
interspecies interaction between organisms
positive regulation of peptidyl-tyrosine phosphorylation
alphav-beta3 integrin-vitronectin complex
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD49e antibody
|Functional Assays 1
|Immunohistology - Frozen
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul of whole blood.
- Histology Positive Control Tissue
- Human Endothelium
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD49e antibody
Shen, C.X. et al. (1991) Antibodies to different members of the beta 1 (CD29) integrins induce homotypic and heterotypic cellular aggregation.
Cell Immunol. 138 (1): 216-28.
McCarty, O.J. et al. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion.
J Thromb Haemost. 2 (10): 1823-33.
Bax, D.V. et al. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells.
J Biol Chem. 279 (21): 22377-86.
Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
Neoplasia. 11: 651-61.
Yao, H. et al. (2010) Increased potency of the PHSCN dendrimer as an inhibitorof human prostate cancer cell invasion, extravasation, and lung colony formation.
Clin Exp Metastasis 27:173–84.
Gassmann, P. et al (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
BMC Cancer. 10: 177.
Orazizadeh, M. et al. (2008) CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
Arthritis Res Ther. 10: R4.