CD49e antibody | JBS5

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Mouse anti Human CD49e

Product Type
Monoclonal Antibody
Clone
JBS5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1187T C F IP 25 µg
MCA1187 C F FN* IP 0.2 mg
Mouse anti Human CD49e antibody, clone JBS5 recognizes the Alpha 5 integrin, also known as CD49e, VLA-5, Fibronectin receptor subunit alpha or Integrin alpha-F. CD49e is a 1049 amino acid ~130kDa single pass type-1 transmembrane glycoprotein containing seven FG-GAP repeats. Mouse anti human CD49e antibody, clone JBS5 been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines (Shen et al. 1991). It also inhibits the adherence of cells to fibronectin.

Removal of sodium azide is recommended prior to use in functional assays.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
Pack Size: 25 µg
Ig concentration 1.0 mg/ml
Pack Size: 0.2 mg
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08648 Related reagents
Entrez Gene
ITGA5 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001726 ruffle
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0035313 wound healing, spreading of epidermal cells
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050900 leukocyte migration
GO:0071062 alphav-beta3 integrin-vitronectin complex
Regulatory
For research purposes only

Applications of CD49e antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays 1
Immunohistology - Frozen 0.5ug/ml 1ug/ml
Immunoprecipitation 1ug/ml 5ug/ml
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul of whole blood.
Histology Positive Control Tissue
Human Endothelium

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Functional Assays

Western Blotting

Product Specific References

References for CD49e antibody

  1. Shen, C.X. et al. (1991) Antibodies to different members of the beta 1 (CD29) integrins induce homotypic and heterotypic cellular aggregation.
    Cell Immunol. 138 (1): 216-28.
  2. McCarty, O.J. et al. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion.
    J Thromb Haemost. 2 (10): 1823-33.
  3. Bax, D.V. et al. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells.
    J Biol Chem. 279 (21): 22377-86.
  4. Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
    Neoplasia. 11: 651-61.
  5. Yao, H. et al. (2010) Increased potency of the PHSCN dendrimer as an inhibitorof human prostate cancer cell invasion, extravasation, and lung colony formation.
    Clin Exp Metastasis 27:173–84.
  6. Gassmann, P. et al (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
    BMC Cancer. 10: 177.
  7. Orazizadeh, M. et al. (2008) CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
    Arthritis Res Ther. 10: R4.